微流控芯片上细胞培养与分析方法研究进展  

Development of Cell Culture and Analysis on Microfluidic Devices

作　　者：林路遥 林金明[1] 

机构地区：[1]清华大学化学系微量分析测试方法与仪器研制北京市重点实验室,北京100084

出　　处：《分析科学学报》2017年第5期707-714,共8页Journal of Analytical Science

基　　金：国家自然科学基金(No.81373373;21575044;21621003)

摘　　要：微流控芯片以其强大的微流体和微小物质控制能力成为研究单细胞、细胞群落乃至生物组织的重要手段。在本篇综述中,我们将以微流控芯片上细胞体外培养模型的建立为主,对近几年来重要的研究工作加以评述,全面地介绍微流控技术在细胞生命科学研究中应用的优势和未来发展方向,具体包括微流控芯片的细胞操控能力、细胞培养微环境的构建以及芯片联用检测手段,希望为从事这一领域研究工作的读者提供一些新的思路。Application of microfluidic technology in cell biological discipline, especially in the study of cellcell interaction and cell signal conducting pathway has become the foreground of academic attention in recent years, assisted by its powerful functions and desire oriented designs. Relevant works have implicated great potential in serious diseases diagnosis, pathogenesis study and drug screening. Benefiting from its ability of micro-fluid and small objects manipulation, microfluidic device is regarded as an ideal candidate for those researches targeting at single cell, small population or even biological tissues. In this review,we will introduce around the theme of building cell in vitro culture model on microfluidic device, and concentrate on important progressions made in the last few years. Microfluidic cell manipulation strategies,compositions of cell culture microenvironment, chip coupled detection methods as well as the advantages and challenges of this technology will be completely reviewed. We hope this short article can provide interested readers and those wish to learn more about microfluidic application in cell biology with knowledge and inspiration.

关 键 词：微流控芯片 细胞分选 细胞三维培养 芯片器官 

分 类 号：O657[理学—分析化学]