不同温度层积处理对北五味子种子休眠过程中种胚后熟的影响  

Effects of Different Temperature Stratification Treatments on Seed Germination of Schisandra chinensis Seed During Dormancy

作　　者：李阳[1] 于锡宏[1,2] 蒋欣梅 王建[2] 

机构地区：[1]东北农业大学农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030 [2]黑龙江省林下经济资源研发与利用协同创新中心,黑龙江哈尔滨150040

出　　处：《北方园艺》2017年第20期140-144,共5页Northern Horticulture

基　　金：国家重点研发计划子课题资助项目(2016YFC0500307-06)

摘　　要：以北五味子种子为试材,采用不同温度层积处理的方法,研究了变温层积处理对北五味子种子休眠过程中种胚后熟的影响。结果表明:持续一定时间(90d)的12℃高温层积处理(T1)和12℃高温层积后结合-4℃低温变温层积处理(T3、T4、T5,处理天数均为90d)均有利于北五味子种胚后熟,使种胚占有率显著增加,而变温层积处理比持续高温处理T1更有利种胚后熟,其中12℃层积60d后再进行-4℃低温层积30d的处理(T5)最佳,其种胚占有率最大,种胚的成熟度最高。随着层积处理天数的增加,种子内的可溶性糖、可溶性蛋白质、脱落酸含量均下降,赤霉素含量表现为先下降后上升趋势,说明种胚的后熟需要消耗更多的可溶性糖、可溶性蛋白质、脱落酸、赤霉素含量的升高有利于解除休眠促进种胚后熟。The seeds of Schisandra chinensis were used as test materials, using the method of different temperature stratification, the effects of temperature stratification in the process of embryo after ripening of seed dormancy of Schisandra chinensis, were studied. The results showed that 12 ℃ stratification for 90 days （T1） and 12 ℃ stratification combined with -4 ℃ stratification （T3, T4, T5 treatment, treatment days were 90 days） were benefit for embryo development of Schisandra chinensis seed, the ratio of embryo sharing were raised after treatment. While the variable temperature stratification was more beneficial for embryo development than 12℃ stratification for 90 days. The development of seed embryo was the best after treated by 12℃ stratification for 60 days combined with -4℃ stratification for 30 days（T5） compared with other treatments. In the progress of breaking dormancy,the content of soluble sugar, soluble protein and abscisic acid were decreased over time, while,the content of gibberellin decreased after an initial increase,which indicated that more soluble sugar and soluble protein be consumed and endogenous hormone such as abscisic acid, gibberellin could speed up embryo maturation and break dormancy of Schisandra chinensis seed.

关 键 词：北五味子 变温层积 呼吸底物 激素 

分 类 号：S567.19[农业科学—中草药栽培]