钛合金表面纳米化对成骨细胞生物学行为的影响  

Effects of Surface Nanocrystallization of Titanium Alloy on Biological Behavior of Osteoblast

作　　者：黄润[1] 张兰[2] 黄雷 胡东[4] 

机构地区：[1]安徽理工大学材料科学与工程学院,安徽淮南232001 [2]西安交通大学金属材料强度国家重点实验室,西安710049 [3]湖北省肿瘤医院胃肠外科,武汉430060 [4]安徽理工大学医学检验中心,安徽淮南232001

出　　处：《中国表面工程》2017年第5期81-88,共8页China Surface Engineering

基　　金：国家自然科学基金(81501598);西安交通大学金属材料强度国家重点实验室开放研究项目(20161805);安徽理工大学引进人才科研启动基金(ZY533)~~

摘　　要：采用表面机械研磨(SMAT)方法对Ti-25Nb-3Mo-3Zr-2Sn(TLM)钛合金进行处理,并将处理前后的钛合金样品表面机械抛光成镜面,制备出Un SMATed及SMATed两组样品。XRD、OM、TEM、AFM及接触角试验结果显示,两组样品表面具有相同的物相组成、相近的拓扑结构及粗糙度,最表层的晶粒尺度分别为(82±9)μm及(48±7)nm,且晶粒细化后的SMATed样品表面具有更好的亲水性;体外试验结果显示,SMATed样品的纳米晶表面能从血清中吸附更高的蛋白量,从而促进其上成骨细胞的黏附、增殖与分化。结果表明,SMAT诱发的表层晶粒细化改善了TLM钛合金表面的细胞生长环境,从而有望应用于新型医用硬组织替代材料的开发。Ti-25Nb-3Mo-3Zr-2Sn （TLM） titanium alloy was treated by surface mechanical attrition treatment （SMAT）, and the treated and untreated titanium alloy were both mechanically polished to mirror finish to fabricate unSMATed and SMATed two groups of samples. XRD, OM, TEM, AFM and contact angle experimental results together reveal that the two kinds of samples possess the same phase composition, similar surface topography and roughness. The grain size in the exposed surface layers of unSMATed and SMATed samples is （82±9） μm and （48±7） nm, respectively. In addition, the SMATed surface with grain size reduces to nanoscale exhibited much better hydrophilicity. In vitro experimental results show that the SMATed sample with a nanograined surface can adsorb more protein from the serum, thus promoting osteoblast adhesion, proliferation and differentiation on its surface compared with the unSMATed surface. The results suggest that SMAT-induced grain refinement improves the living condition of osteoblast on TLM alloy surface, and has potential application in developing new biomedical hard-tissue substitutes.

关 键 词：钛合金 晶粒细化 蛋白吸附 细胞黏附 增殖 

分 类 号：TG174.44[金属学及工艺—金属表面处理] TG580.68[金属学及工艺—金属学]