海洋真菌Aspergillus sp. SCS-KFD03的化学成分分析  

Analysis of Chemical Constituents from the Marine-derived Fungus Aspergillus sp. SCS-KFD03

作　　者：张宇 孔凡栋[2] 马青云[2] 谢晴宜[2] 黄圣卓[2] 周丽曼[2] 戴好富[2] 邓世明[1] 赵友兴[2] 

机构地区：[1]海南大学海洋学院/热带生物资源教育部重点实验室,海口570228 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《热带生物学报》2017年第3期248-254,共7页Journal of Tropical Biology

基　　金：公益性行业(农业)科研专项(201303117);国家自然科学基金青年科学基金项目(41606088);中央级公益性科研院所基本科研业务费专项-中国热带农业科学院院级创新团队项目(17CXTD-15);天津大学-海南大学协同创新基金资助

摘　　要：为了研究海洋真菌Aspergillus sp.SCS-KFD03次级代谢产物的活性成分,采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等技术从其发酵液中分离得到7个化合物,运用各种波谱方法鉴定为3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one(1);3β,4α-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one(2);麦角甾醇(22E,24R)-ergosta-5,7,22-trien-3β-ol(3);leporin B(4);astellolide G(5);astellolide B(6)和astellolide A(7),并测定化合物的抗氧化活性、乙酰胆碱酯酶和α-糖苷酶抑制活性。结果表明:化合物2,3,5和6具有一定的抗氧化活性,化合物2具有较弱的乙酰胆碱酯酶抑制活性,化合物4具有强于阳性药阿卡波糖的α-糖苷酶抑制活性。A strain SCS-KFD03 of AspergiUus sp. was isolated from Sipunculus nudus collected from Haikou Bay, and fermented. Active constituents were isolated and purified from the fermentation broth and hyphae of the strain SCS-KFD03 by using various chromatographic techniques, including Silica gel, Sephadex LH-20 column and HPLC chromatography. Seven compounds （ 1-7 ） were extracted from the secondary metabolites of the strain SCS-KFD03 and identified as 3β,5α-dihydroxy-（22E,24R）-ergosta-7,22-dien-6-one（ 1 ）, 3β,4α-dihydroxy-26- methoxyer gosta-7,24 （28） -dien-6-one （2）, （22E, 24R） -ergosta-5,7,22-trien-3β-α （ 3 ）, leporin B （4）, as- tellolide G （5）, astellolide B （6） and astellolide A （7）. The bio-activity evaluation of these compounds showed that compounds 2, 3,5 and 6 had a certain antioxidant activity towards DPPH free radical. The compound 2 also exhibited a weak inhibitory activity against acetyl cholinesterase, and the compound 4 had a higher inhibitory ac- tivity against α-glucosidase than the positive control acarbose.

关 键 词：海洋真菌 曲霉属 化学成分 生物活性 

分 类 号：R284.1[医药卫生—中药学]