普通大蓟马不同地理种群的分子鉴定及酶活测定  

Molecular Identification and Enzyme Activity Determination of Different Geographical Populations of Megalurothrips usitatus(Bagnall)

作　　者：苏美凤 孙靖雨 胡坚 吴迪 庙浩 范咏梅 

机构地区：[1]海南大学热带农林学院,海口570228

出　　处：《热带生物学报》2017年第3期364-370,共7页Journal of Tropical Biology

基　　金：公益性行业(农业)科研专项经费项目"南繁区生物安全监测预警及控制关键技术研究与示范"(201403075);海南省教育厅项目(HNJG2014-07);海南省研究生创新科研课题(Hys2016-26)

摘　　要：普通大蓟马Megalurothrips usitatus(Bagnall)是影响海南豇豆品质的重要害虫之一。笔者在采集不同地理种群的基础上,用试剂盒法提取其总DNA,对mt DNA COⅠ基因序列进行测序分析,并构建系统发育树;用分光光度计法进行酶活比较分析。结果表明,普通大蓟马不同地理种群之间的遗传距离非常小,并没有随地理位置的变化产生较大的遗传分化;抗氧化酶系活力测定中,不同地理种群的普通大蓟马GST活力差异较大,SOD活力不存在显著性差异,个别地理种群CAT活力与其他地理种群存在显著性差异。Bean flower thrips, Megalurothrips usitatus （ Bagnall）, is one of the major pests in cowpeas grown in Hainan, China, affecting the quality of cowpeas. From different geographical populations of M. usitatus collected, the total DNA of M. usitatus was extracted by using TIANamp Genmmic DNA Kit, and the mtDNA COI gene sequence was sequenced, based on which a phylogenetic tree was constructed. The enzyme activity was de- termined by using spectrophotometer. The results showed that the genetic distance between the different geo- graphical populations was very small, and that there was no significant genetic differentiation with the change of geographical location. Determination of antioxidant enzyme activity showed significant difference in glutathione-S- Transferase （GST） activity between different geographic populations. There was no significant difference in SOD activity between different geographic populations. There was significant difference in CAT activity between indi- vidual populations and other geographic populations.

关 键 词：普通大蓟马 分子鉴定 酶活 地理种群差异 

分 类 号：S186[农业科学—农业基础科学]