转移生长因子β_1对体外培养破骨细胞功能影响的实验研究  

Effects of Transforming Growth Factor β_1 on the Osteoclast-like Cells in vitro

作　　者：鲁道海[1] 陈建庭[2] 金大地[2] 郑晓明[1] 郭振河[1] 陈映红[1] 

机构地区：[1]解放军第161中心医院骨科,武汉市430010 [2]广州南方医院骨质疏松研究中心,510515

出　　处：《中国矫形外科杂志》2002年第9期898-900,共3页Orthopedic Journal of China

摘　　要：目的 :探讨转移生长因子 β1(TGF β1)对体外培养破骨细胞功能影响的机制。方法 :酶动力学法测定培养基中酸性磷酸酶和抗酒石酸酸性磷酸酶活性 ;共聚焦激光扫描显微镜观察体外培养破骨细胞内氢离子随TGF β1浓度的变化情况 ;LeicaQuantimet 5 0 0图像分析系统对骨吸收陷窝进行图像分析 ;扫描电镜观察骨吸收陷窝变化。结果 :随着TGF β1浓度的升高 ,处理组与对照组ACP和TRAP的活性分别从 (1.71± 0 .3 3U) /L和 (1.41± 0 .2 9)U /L降低到 (1.3 2± 0 .2 1)U/L和 (1.0 1± 0 .11)U /L(均为P <0 .0 1) ,骨吸收陷窝的数目与面积从 (16.67± 1.3 5 )个 /片和 (5 82 .2 4± 178.68) μm2 减少到 (13 .2 9± 1.47)个 /片与 (4 4 2 .3 8± 14 8.2 7) μm2 ,处理组与对照组比较差异具有显著性 (P <0 .0 1)。破骨细胞相对荧光值无显著性差异 ,表明TGF β1对体外培养破骨细胞分泌H+ 无明显影响。结论 :TGF β1虽然不能抑制氢离子释放 ,但能通过改变酸性磷酸酶和抗酒石酸酸性磷酸酶活性 。Objective:To study the effects of transforming growth factor β 1 on the osteoclast like cell in vitro.Methods:The acid phosphatase(ACP)and tartrate resistant acid phosphatase(TRAP)activities were measured kineticly and the introcellular hydrogen ions were calculated on the confocal laser scanning microscope.The area and number of the resorption pits were determined with the Leica Quantimet 500 system Scanning.Electronicmicroscopy shows the area of bone resorption pits.Results:The ACP and TRAP activity had difference( P< 0.01)when the density of transforming growth factor β 1 was 10 -2 ng/ml,10 -1 ng/ml,1ng/ml and 5ng/ml.The area and number of the resorption pits were significant( P< 0.01)when the density of transforming growth factor β 1 was 10 -2 ng/ml,10 -1 ng/ml,1ng/ml and 5ng/ml.The hydrogen ions release could not significantly be inhabitted by transforming growth factor β 1.Conclusions:The transforming growth factor β 1 could not inhabit hydrogen ions,but could decrease osteoclastic bone resorption due to the TRAP.

关 键 词：转移生长因子Β1 破骨细胞 抗酒石酸酸性磷酸酶 酸性磷酸酶 骨吸收 

分 类 号：R68[医药卫生—骨科学]