吲哚美辛亲水凝胶贴剂定量方法与前处理提取工艺正交优化  

Optimization of Extraction Process of Quantitative Method and Pretreatment of Indomethacin Hydrophilic Gel Patch by Orthogonal Design

作　　者：郭文韬[1] 谢伟杰[1,2] 张永萍 徐剑[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国药业》2017年第20期13-17,共5页China Pharmaceuticals

基　　金：国家苗药工程技术研究中心项目[2014FU125Q09];贵州省科技厅"百层次人才"培养计划项目[黔科合人才[2015]4030号];贵州省教育厅中药民族药(苗药)新剂型新制剂工程研究中心项目[黔教合KY字[2014]22号];贵州省普通高等学校中药民族药制剂重点实验室[黔教合KY字[2016]005]

摘　　要：目的优化吲哚美辛亲水凝胶贴剂的提取工艺与含量测定方法。方法利用高效液相色谱(HPLC)-工作曲线进行定量,通过单因素考察与正交设计优化贴剂的提取方法、提取溶剂、时间与预处理方法。结果最终确定贴剂的提取条件是将贴剂膏体剪碎后,50 mL甲醇提取90 min,提取1次,或用25 mL甲醇提取60 min,提取2次。结论优化的贴剂中药物提取工艺条件稳定、可靠,可为贴剂的质量评价提供良好的方法。Objective To optimize the extraction process and content determination method of Indomethacin Hydrophilic Gel Patch. Methods Quantitative analysis was performed by using HPLC - working curves,the extraction method,extracting solvent,time and pretreatment method were extracted by means of single factor and orthogonal design optimization. Results Finally,the extraction conditions of the patch were：after the patch paste was cut and broken,50 mL methanol was extracted for 90 min and extracted 1 time,or 25 mL methanol was extracted for 60 min and extracted 2 times. Conclusion Drug extraction process conditions in optimized patches is stable and reliable,which provides a good method for the quality evaluation of the patch.

关 键 词：正交设计 提取工艺 吲哚美辛 含量测定 亲水凝胶 贴剂 

分 类 号：TQ460.6[化学工程—制药化工] R944.2[医药卫生—药剂学]