靶向hnRNP K基因siRNA的筛选及其对精原细胞存活的影响  

Screening of siRNA Targeted for hnRNP K Gene and Its Effect on the Spermatogonium Survival

作　　者：徐海霞[1] 李瑞[1] 王素莹 张凯丽[1] 张朋朋 陈磊[1] 徐永杰[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2017年第4期561-566,共6页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(U1204326);河南省青年骨干教师资助计划项目(2015GGJS-139);河南省高等学校重点科研项目(18B230011)

摘　　要：利用免疫细胞荧光、RNA干涉(RNA interfere,RNAi)、qRT-PCR、Western blot及细胞计数等方法,设计合成三对小分子干扰RNA(small interfering RNA,siRNA)(siRNA-hnRNP K-1,2,3),对小鼠精原细胞中内源性的不均一性核糖核蛋白K(Heterogeneous ribonucleoproteins K,hnRNP K)进行敲低并对其沉默效率进行筛选,研究其对小鼠精原细胞存活的影响.结果表明,siRNA-hnRNP K-3对小鼠精原细胞具有80%以上的沉默效率,转染后发现细胞数目明显减少,说明沉默hnRNP K基因的表达对小鼠精原细胞的存活有显著影响.Through the immune cell fluorescence,RNAi（RNA interfere）,qRT-PCR,Western blot and cell counting methods,three pairs of siRNA（small interfering RNA）（siRNA-hnRNP K-1,2,3）were designed and synthesed to screen the silence efficiency that the endogenous hnRNP K of spermatogonium in mice were knockdown,and then studied the effect on spermatogonium survival.Results showed that the siRNA-hnRNP K-3had silence efficiency more than 80%,and the cell number decreased significantly from further transfection to spermatogonium in mice.The results showed that the hnRNP K gene knockdown had a significant effect on the survival of spermatogonium in mice.

关 键 词：不均一性核糖核蛋白K 精原细胞 小鼠 精子发生 

分 类 号：S823.2[农业科学—畜牧学] Q959.842.03[农业科学—畜牧兽医]