桑黄扩大培养过程中黄酮产量变化研究  

Study on the Change of Flavonoids Production in the Expanding Culture Process of Phellinus igniarius

作　　者：杜睿绮 王芯蕾 许谦[2] 

机构地区：[1]菏泽一中宏志级部 [2]菏泽学院农业与生物工程学院生命科学系,山东菏泽274000

出　　处：《安徽农业科学》2017年第29期109-111,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省重点研发项目(2016GNC113019);菏泽市科技发展计划项目(2012N002)

摘　　要：[目的]对桑黄进行液体培养基的扩大培养,分析扩大培养过程中黄酮产量的变化。[方法]250、500、1 000 m L 3种锥形瓶分别装液体培养基150、300、600 m L,在28℃、180 r/min条件下培养。采用有机溶剂浸提法提取黄酮,分光光度法测定黄酮产量。[结果]在扩大培养过程中,桑黄胞内和胞外黄酮产量均呈现先下降再上升的趋势。[结论]该研究结果对桑黄胞内和胞外黄酮的生产、提取及应用具有现实的指导意义。[Objective]The research aimed to expand the culture medium of Phellinus igniarius and analyze the change of flavonoid production during the culture process. [Method]150,300,600 m L liquid medium were stalled in 250,500,1 000 m L flasks respectively and incubated under 28 ℃,180 r/min conditions. Organic solvent extraction was used to extract flavonoids and spectrophotometry was used to measure flavonoids production. [Result]In the process of expanding the culture,the intracellular and extracellular flavonoids production of Phellinus igniarius showed a tendency to decrease first and then rise. [Conclusion]The results of this study have guiding significance for the production,extraction and application of intracellular and extracellular flavonoids from Phellinus igniarius.

关 键 词：桑黄 液体培养基 扩大培养 黄酮产量 

分 类 号：S567.3[农业科学—中草药栽培]