EPCs联合胚胎鼠跖骨血管形成模型建立可行性的初步研究  

Preliminary Study on the Feasibility of Establishing the Angiogenesis Assay Model by Co-culturing EPCs and Fetal Metatarsal

作　　者：万龙飞[1] 张宏伟[1] 王小义[1] 葛权虎 程文哲 董丹 陈聪哲 陈雪玲 吴向未[1] 

机构地区：[1]石河子大学医学院第一附属医院普外科,新疆石河子832008 [2]石河子大学医学院免疫学教研室,新疆石河子832002

出　　处：《农垦医学》2017年第4期289-293,共5页Journal of Nongken Medicine

基　　金：国家自然科学基金(31271458);人力资源和社会保障部留学回国人员科技活动项目(RSLX201201);新疆生产建设兵团应用基础研究计划(2016AG019);石河子大学高层次人才科研启动项目(RCZX201538)

摘　　要：目的:通过对胚胎鼠跖骨周围芽生的管状结构面积进行量化,探讨加入血管形成模型的相对最适合的EPCs细胞量,并为EPCs联合胚胎鼠跖骨血管形成模型奠定实验基础。方法:改良差时贴壁培养法提取EPCs,与胎鼠跖骨共培养14天后,进行CD31免疫染色,用IPP对染色阳性面积进行量化分析。结果:不同EPCs细胞数量处理组的胎鼠跖骨,可根据其周围芽生的管状结构面积的量化来分析模型中EPCs的活性;加入数量为5×10~4个EPCs时,胎鼠周围芽生的管状结构的面积最大(P<0.05)。结论:初步构建了EPCs联合胚胎鼠跖骨血管生成实验模型,并探讨了模型中EPCs的最适数量。Objective： By quantificating the endothelial sprouting tube-like structures from fetuses mouse metatarsals, we try to find the most suitable number of EPCs,and lay a experimental foundation for the EPCs and Fetal mouse metatarsal co-culture angiogenesis assay.Methods： EPCs were isolated from murine bone marrow of tibia and femur, the third generation of EPCs were co-culured with fetal mouse metatarsals forl4 days ,then immunostained the endothelial sprouting tube-like structures from the metatarsals with CD31,the positive staining outgrowth tubelike structures were quantified by computerized image analysis software （Image-Pro Plus 6.0）.Results： The cellular activities were analyzed by quantificating the endothelial sprouting tube-like structures from fetuses mouse metatarsals in experiment groups treated with various number of EPCs ,resulting that the maximum outgrowth structures aera are the groups with 5×10^4 EPCs （P〈0.05）.Conclusion：We successfully constructed the angiogenesis assay model by co-culturing EPCs and fetal metatarsal ,and studied the optimal number of the EPCs in the assay model.

关 键 词：内皮祖细胞 胚胎鼠跖骨 血管生成 实验模型 

分 类 号：Q813[生物学—生物工程]