aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用  

The role of aptamer-siRNA nucleic acid compound in promoting the apoptosis of K562 cells

作　　者：卢建华[1] 崔照琼 赵召霞[1] 吕颖[1] 许怡[1] 

机构地区：[1]石家庄市第五医院检验科,河北石家庄050021 [2]昆明医科大学海源学院细胞生物学暨医学遗传学教研室,云南昆明650106

出　　处：《西安交通大学学报（医学版）》2017年第6期862-865,871,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的观察aptamer-siRNA核酸复合物对人慢性粒细胞白血病(CML)细胞株K562细胞凋亡的影响,并探讨其作用机制。方法使用不同浓度的aptamer-siRNA溶液转染K562细胞,噻唑蓝(MTT)法检测aptamer-siRNA对K562细胞增殖的影响,AnnexinV/PI双染法检测aptamer-siRNA对K562细胞凋亡的影响,Western blot和RT-PCR法检测aptamer-siRNA对K562细胞bcl-2、Bax和caspase-3蛋白及mRNA表达的影响。结果与对照组相比,转染aptamer-siRNA后K562细胞增殖受到明显抑制,K562细胞的早期凋亡率明显增加,细胞中bcl-2蛋白和mRNA的表达水平明显降低,Bax和caspase-3蛋白和mRNA的表达水平明显升高,差异均具有统计学意义(P<0.05)。aptamersiRNA核酸复合物浓度(50~250μmol/L)对bcl-2、Bax和caspase-3 mRNA的影响具有明显的量效关系。结论aptamer-siRNA核酸复合物能够通过促使bcl-2基因减少和Bax、caspase-3基因增长,进而促进K562细胞凋亡。Objective To observe the effect of aptamer-siRNA nucleic acid compound on the apoptosis of K562 cells in human chronic myelogenous leukemia（CML）and explore its acting mechanisms.Methods K562 cells were transfected with different concentrations of aptamer-siRNA solution.The effects of aptamer-siRNA on the proliferation and apoptosis of K562 cells were detected by MTT method and AnnexinV/PI double staining method,respectively.The effects of aptamer-siRNA on the expressions of bcl-2,Bax and casepase-3 at protein and mRNA levels in K562 cells were detected by Western blot and RT-PCR method,respectively.Results Compared with the control group,the proliferation of K562 cells was significantly inhibited,early apoptosis rate of K562 cells increased significantly,the expression levels of bcl-2 protein and mRNA were significantly decreased,while the expression levels of Bax and caspase-3 protein and mRNA were significantly increased after transfection with aptamer-siRNA（P〈0.05）.Aptamer-siRNA nucleic acid complex at the concentration of 50-250μmol/L）had a significant dose-effect relationship on bcl-2,Bax and caspase-3 mRNA.Conclusion Aptamer-siRNA nucleic acid compound can promote the decreased number of bcl-2 gene and the growth of Bax and caspase-3 genes,thus promoting the apoptosis of K562 cells.

关 键 词：慢性粒细胞白血病 aptamer-siRNA核酸复合物 K562细胞 细胞凋亡 

分 类 号：R733.72[医药卫生—肿瘤]