空心莲子草总皂苷对HepG2.2.15细胞系HBsAg和HBeAg表达及HBV-DNA含量的影响  

Effects of Saponins Extracted from Alternanther philoxeroides on HBsAg and HBeAg Expressions and HBV-DNA Contents in HepG2.2.15 Cells

作　　者：武谦虎 徐卫东[2] 李伟东[3] 徐璐 凌美 吴玉华 WU Qian-hu XU Wei-dong LI Wei-dong XU Lu LING Mei WU Yu-hua(Zhenjiang Third Hospital Affiliated to Jiangsu University, Zhenjiang 212000 School of Pharmacy, Jiangsu University, Zhenjiang 212013 School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023)

机构地区：[1]江苏大学附属镇江三院,镇江212000 [2]江苏大学药学院,镇江212013 [3]南京中医药大学药学院,南京210023

出　　处：《药学与临床研究》2017年第5期381-384,共4页Pharmaceutical and Clinical Research

基　　金：镇江市2012年科技支撑-社会发展项目(SH2012048)

摘　　要：目的:研究空心莲子草总皂苷对乙肝病毒的抑制作用。方法:应用光密度测定法和细胞培养技术,研究空心莲子草总皂苷对HepG2.2.15细胞系HBsAg与HBeAg表达的影响。结果:96孔板试验:实验第3、6日,空心莲子草总皂苷对HBsAg和HBeAg表达均有明显的抑制作用(与对照组比较P<0.01);同时采用定量PCR方法检测,空心莲子草总皂苷(200 mg·L-1)可使培养基中HBV-DNA转阴。结论:空心莲子草总皂苷有较强的体外抗乙肝病毒作用。Objective： To study the anti-HBV effect of saponins extracted from Alternanther philoxeroides. Methods： Optical density mensuration and cell culture were used in the research of the influence of Alternanther philoxeroides saponins on expressions of HBs Ag and HBe Ag and HBV-DNA content in Hep G2.2.15 Cells. Results： Compared with that of the control group, the saponins showed a significant inhibitory effect on the expressions of HBs Ag and HBe Ag in Hep G2.2.15 cells（P0.01）; Saponins extracted from Alternanther philoxeroides（200 mg·L-1） converted the HBV-DNA in Hep G2.2.15 cells into negative.Conclusion： The Alternanther philoxeroides saponins may have a strong anti-HBV action in vitro.

关 键 词：空心莲子草 总皂苷 HEPG2.2.15细胞 HBSAG HBeAg HBV-DNA 

分 类 号：R963[医药卫生—微生物与生化药学]