不同夹带剂条件下超临界CO2流体萃取油用牡丹及成分分析  

Supercritical CO_2 Extraction and Analysis of Fatty Acid Composition of Oil Peony in Different Cosolvent Condition

作　　者：华梅[1,2] 马惠芬[1,2] 陈中华[1,2] 原晓龙[1,2] 杨卫[1,2] 呼延丽[1,2] 杨宇明 王娟[1,2] 

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省森林植物培育与开发利用重点试验室,国家林业局云南珍稀濒特森林植物保护和繁育重点试验室,云南昆明650201

出　　处：《西南林业大学学报（自然科学）》2017年第5期198-202,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省科技计划项目(2015IA005)资助;云南省应用基础研究计划青年项目(2014FD069)资助;云南省应用基础研究重点项目(2013FA054)资助;云南省重点实验室开放基金项目资助

摘　　要：采用超临界CO_2流体萃取油用牡丹籽油,用气质联用仪(GC-MS)对牡丹油中脂肪酸成分进行分析,探讨不同夹带剂及用量条件下对油用牡丹籽油脂提取率的影响。结果表明:在压力35 MPa、CO_2流量为25 g/min、时间2 h、温度40℃的萃取条件下,不同夹带剂使超临界CO_2流体萃取油用牡丹带壳油脂提取率均有提高,乙酸乙酯提高了16.72%,石油醚提高了6.52%,正己烷提高了12.85%;综合考察3种夹带剂及其用量,乙酸乙酯的提高效果最好,其最佳用量为10%;牡丹油脂肪酸成分分析结果显示,牡丹油不饱和脂肪酸含量丰富,尤以α-亚麻酸的含量最高,占总脂肪酸含量的78.95%。The way of supercritical CO2 fluid extraction was used in oil peony, and the effects of different conditions of different kinds of cosolvents and dosage on the yield of extraction of oil peony were studied. GC-MS technology was used for analysis of fatty acids composition in the oil peony. The result shows that at the extraction condition： 35 MPa for pressure, 25 g/rain for CO2 flow rate, 2 h for extraction time and 40 ℃ for temperature, the oil yield with shell has been increased 16. 82% after using ethyl acetate as cosolvent. As well the oil yield with shell has been increased 6.55% after using petroleum ether as cosolvent. And the oil yield with shell has been increased 12. 89% after using n-hexane as cosolvent. As can be seen from the results the oil yields with shell all have improved after using of cosolvents, and ethyl acetate has shown the optimum efficiency in oil yields in supercritical CO2 fluid extraction. Comprehensively studied the dosages of 3 cosolvents, the optimal dosage of eosolvents was 10% of CO2 flow rate of ethyl acetate. Peony oil fatty acid composition analysis showed that, oil peony riched in unsaturated fatty acid, especially α-linolenic acid, which take up 78.95% content of the total fatty acid.

关 键 词：牡丹 超临界CO2流体 GC-MS 脂肪酸 夹带剂 

分 类 号：S789.7[农业科学—林学]