UV+MW复合诱变结合抗性突变筛选替考拉宁高产菌株  

Screening of high teicoplanin producing strain by UV + MW mutagenesis combining resistance screening

作　　者：王德森[1] 张祝兰[1] 任林英[1] 唐文力[1] 杨煌建[1] 邱观荣[1] 连云阳[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《工业微生物》2017年第5期21-24,共4页Industrial Microbiology

基　　金：福建省科技重大项目(2014YZ0004-2);福建省化药技术重大研发平台(No.2014Y2001);福州市生物制药行业技术创新中心(No.2016-PT-36);福建省公益类科研院所专项(2017R1009-13)

摘　　要：采用微波照射(MW)、紫外照射(UV)和MW+UV处理技术,对替考拉宁产生菌AT-92的孢子进行诱变,诱变处理的孢子悬液涂布在含替考拉宁致死浓度的培养基平板上培养,获得替考拉宁抗性突变株,通过摇瓶发酵对替考拉宁抗性基因突变株进行筛选,获得一株遗传性状稳定的替考拉宁高产菌AT 92-52-37菌株,其产替考拉宁能力比出发菌株的提高5倍。After treated by microwave irradiation（MW）,ultraviolet radiation（UV） and MW ＋ UV respectively,spore suspension of teicoplanin producing strain Actinoplanes teichomyceticus AT-92 was spreaded on the regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked out and screened by shake flask fermentation. And then a high-yield mutant strain AT 92-52-37 was obtained with genetic stability. As a result,the teicoplanin production capacity of this high-yield mutant was 5 times higher than that of the original strain.

关 键 词：替考游动放线菌 抗性突变筛选 复合诱变 替考拉宁 

分 类 号：Q93[生物学—微生物学]