小麦诱导抗性基因TaWIR1b的克隆及表达分析  

Cloning of the wheat induced resistance gene TaWIR1b and expression analysis

作　　者：王俊美[1] 徐飞[1] 宋玉立[1] 李亚红[1] 刘露露[1] 韩自行[1] 

机构地区：[1]河南省农业科学院植物保护研究所,农业部华北南部作物有害生物综合治理重点实验室,郑州450002

出　　处：《植物保护》2017年第5期150-153,共4页Plant Protection

基　　金：河南省小麦产业技术体系(S2010-01-05);河南省基础与前沿技术研究计划项目(142300413221);河南省农业科学院自主创新基金

摘　　要：全蚀病是小麦上一种重要的土传病害。选育和种植抗病品种是防治小麦全蚀病的根本途径,抗病基因研究是抗病育种的基础性工作。根据基因TaWIR1b(Accession no.M94959.1)的全长序列设计引物扩增‘新农19’的cDNA,获得了完整ORF,编码85个氨基酸残基,比对后发现与TaWIR1b序列同源性达100%。根据获得的TaWIR1b基因全长序列设计定量引物,分析TaWIR1b在全蚀菌胁迫条件下不同互作模式的表达特征。结果表明接种全蚀病菌后抗病小麦品种‘新农19’中TaWIR1b基因被诱导表达,接菌后3d达到峰值143.97,感病品种‘新麦19’中峰值出现在接菌后8d,表达量仅为对照的4.22倍,提示该基因可能参与小麦对全蚀病的抗病过程。Take-all disease,caused by Gaeumannomyces graminis var.tritici（Ggt）,is one of the important soilborne diseases of wheat.It is the fundamental way to breed and plant resistant varieties for preventing the disease,and research on resistance genes is the foundation for resistance breeding.In this study,a complete ORF（open reading frame）,with100% homology with TaWIR1 b from GenBank,was obtained by amplifying cDNA of‘Xinnong19＇using primers designed based on TaWIR1 b sequence（Accession no.M94959.1）.Primers were synthesized for analyzing the transcription patterns of the gene by quantitative PCR in different wheat-pathogen interaction patterns induced by Ggt.The results indicated that TaWIR1 b was induced by Ggt up-regulation expression in resistant cultivar‘Xinnong19＇,with a peak value of 143.97 three days post inoculation,but the peak value was4.22 at 8 d post inoculation in susceptible cultivar‘Xinmai19＇.It indicates that the gene TaWIR1 b might be involved in the course of resistant response to take-all disease.

关 键 词：小麦全蚀病 TaWIR1b 基因克隆 基因表达分析 

分 类 号：S512.1[农业科学—作物学]