散结通络方对缺血性肾病晚期大鼠MMP-2、TIMP-2表达的影响  

Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2,tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage

作　　者：王蕊 陈桂梅 李伟 刘兰英 李娇艳 李根茂[2] 葛东宇[2] 王耀献[3] WANG Rui CHEN Guimei LI Wei LIU Lanying LI Jiaoyan LI Genmao GE Dongyu WANG Yaoxian(Department of Nephrology, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China Basic Research Center, Beijing University of Chinese Medicine, Beijing 100029, China Department of Nephrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China)

机构地区：[1]北京中医药大学第三附属医院肾病科,北京100029 [2]北京中医药大学基础科研中心,北京100029 [3]北京中医药大学东直门医院肾病科,北京100700

出　　处：《中国医药导报》2017年第29期24-27,F0003,共5页China Medical Herald

基　　金：北京中医药大学基本科研业务费项目面上项目(2015-JYB-JSMS145)

摘　　要：目的探讨散结通络方对缺血性肾病晚期大鼠肾组织基质金属蛋白酶2(MMP-2)、基质金属蛋白酶组织抑制因子2(TIMP-2)表达的影响。方法将SD雄性大鼠按随机数字表法分为假手术组、模型组(均给予生理盐水3 m L/d)、药物组[散结通络方10.85 g/(kg·d)],采用单侧肾动脉不全结扎术建立模型。于实验第28天检测大鼠24 h尿蛋白定量及血肌酐。处死大鼠,取肾组织做光镜检查,观察各组大鼠肾脏形态学改变;免疫组化法检测肾组织MMP-2、TIMP-2表达。结果光镜下模型组和药物组大鼠的肾小球及肾小管间质均有损伤。与假手术组比较,模型组和药物组肾小球硬化指数评分及肾小管间质损伤评分均明显升高(P<0.01),而药物组较模型组降低(P<0.01)。与模型组比较,药物组MMP-2表达升高(P<0.01),24 h尿蛋白定量、血肌酐及TIMP-2表达降低(P<0.01),且两组均高于假手术组(P<0.01)。结论散结通络方可能通过上调MMP-2、下调TIMP-2在肾组织中的表达对缺血性肾病晚期病变起防治作用。Objective To investigate the effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2 （MMP-2）, tssue inhibitor of metalloproteinase-2 （TIMP-2） of nephridial tissue in rats with ischemic nephropathy of advanced stage. Methods Male SD rats were randomly divided into sham operation group, model group （both groups were given normal saline 3 mL/d）, drug group [Sanjie Tongluo Decoction 10.85 g/（kg·d）] by random number table method. The unilateral renal artery insufficiency ligation was used to establish animal model. In the 28th day of experiment, the levels of 24 hours urinary protein and creatinine of rats were detected. The rats were put to death and nephridial tissue was collected to take light microscope examination, the changes of renal morphology in all groups were observed.Immunohistochemistry was used to detect the expression of MMP-2 and TIMP-2 of nephridial tissue. Results Under the light microscope, the glomerulus and tubulointerstitium in the model group and drug group had damage. Compared with sham operation group, the scores of glomerulosclerotie index and tubulointerstitial lesion in the model group and drug group were all increased （P 〈 0.01）, which of drug group were lower than those of model group （P 〈 0.01）. Compared with model group, the expression of MMP-2 in drug group was increased （P 〈 0.01）, 24 hours urinary protein, creati- nine and the expression of TIMP-2 were decreased （P 〈 0.01）, which of both groups were higher than those of sham operation group （P 〈 0.01）. Conclusion Sanjie Tongluo Decoction can prevent the lesions of ischemic nephropathy of advanced stage by up-regulating the expression of MMP2 and down-regulating the expression of TIMP-2.

关 键 词：散结通络方 缺血性肾病 基质金属蛋白酶2 基质金属蛋白酶组织抑制因子2 大鼠 

分 类 号：R692[医药卫生—泌尿科学]