自发性高血压大鼠心肾交感神经的分布状况  

Distribution of heart and kidney sympathetic nerves in spontaneous hypertension rats

作　　者：郭建强[1] 孙鹏[2] 李素娟[3] 赵鹏伟[2] 牛燕[2] 张烜[2] 

机构地区：[1]内蒙古医科大学附属医院心内科,内蒙古呼和浩特010050 [2]内蒙古医科大学微生物与免疫实验中心,内蒙古呼和浩特010050 [3]内蒙古医科大学附属医院消化内科,内蒙古呼和浩特010050

出　　处：《中国现代医学杂志》2017年第24期7-10,共4页China Journal of Modern Medicine

基　　金：内蒙古自治区自然科学基金博士基金(No:2014BS0806)

摘　　要：目的探讨自发性高血压大鼠心脏及肾脏交感神经的分布状况。方法选取6及12周龄的自发性高血压大鼠(SHR)和相应周龄的同源正常血压大鼠(WKY),以儿茶酚胺合成限速酶酪氨酸羟化酶(TH)的表达作为交感神经分布的标志,采用免疫组织化学检测TH,尾套法检测大鼠血压及心率。结果 6及12周龄的SHR血压及心率高于相应周龄的WKY鼠。6及12周龄的SHR心脏及肾脏TH的表达高于相应周龄的WKY鼠。SHR 12周龄时肾脏TH的表达高于6周龄,心脏TH的表达在2个周龄组差异无统计学意义。结论 SHR心及肾脏交感神经纤维分布密度增加,并且随着高血压的进展,肾脏交感神经纤维分布密度有增加的趋势。Objective To investigate the cardiac and nephrological distribution of sympathetic nerves inspontaneous hypertension rats （SHR）. Methods Heart and liver tissues were harvested from 6-week and 12-week old SHR and homogenous Wistar-Kyoto rats （WKY）. Immunohistochemistry staining of Tyrosine hydroxy原lase （TH）, a catecholamine synthesis rate-limiting enzyme, was performed as an indication of sympathetic nerves.Blood pressure and heart rate were measured by tail-cuff method. Results Both blood pressure and heart rateof 6-week and 12-week old SHR were significantly increased compared with those of WKY. Expression levelsof TH in heart and kidney of 6-week and 12-week old SHR were significantly higher than those of WKY. InSHR groups, TH expression in kidney of 12-week upregulated significantly when compared with that of the 6-week group, while there was no difference in terms of hepatic TH between 6-week and 12-week old ones.Conclusion The density of sympathetic nerves is much higher in SHR than that in WKY; nephrological sympathetic nerves were increased in time-dependent manner alongside the progression of hypertension.

关 键 词：高血压 交感神经 心脏 肾脏 

分 类 号：R544[医药卫生—心血管疾病]