Graves病患者血清TLR4、NF-κB和Treg/Th17相关细胞因子表达变化及意义  

Involvement of circulating TLR4, NF-κB and Treg/Th17 related cytokines in Graves' disease

作　　者：王建国[1] 史春云[2] 丑广程[1] 陈占良[1] 冯惠清[1] 王淑仙[1] 

机构地区：[1]河北大学附属医院检验科,河北保定071000 [2]河北省保定市儿童医院内三科,河北保定071051

出　　处：《中国现代医学杂志》2017年第24期58-61,共4页China Journal of Modern Medicine

摘　　要：目的探讨Graves病患者血清Toll样受体4(TLR4)、核因子-κB(NF-κB)和调节性T细胞/辅助性T细胞17(Treg/Th17)相关细胞因子表达变化及意义。方法选取62例初步确诊为Graves病的患者和45例健康者(对照组),采用电化学发光法检测血清中促甲状腺素(TSH)、游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)和促甲状腺素受体抗体(TRAb)水平,利用双抗体酶联免疫吸附试验(ELISA法)对血清中TLR4、NF-κB、TGF-β_1、IL-10、IL-6和IL-17水平进行检测。结果 Graves病患者血清TSH水平低于对照组,而血清FT3、FT4和TRAb水平则高于对照组,差异有统计学意义(P<0.05);Graves病患者血清TLR4、NF-κB、IL-10、IL-6和IL-17水平均高于对照组,而TGF-β_1水平则低于对照组,差异有统计学意义(P<0.05);Pearson相关分析显示,Graves病患者血清TSH与TLR4、NF-κB、IL-10、IL-6和IL-17均呈负相关(P<0.05),而与TGF-β_1呈正相关(P<0.05),血清FT3和FT4均与TLR4、NF-κB、IL-10、IL-6和IL-17呈正相关(P<0.05),而与TGF-β_1呈负相关(P<0.05);Graves病患者血清TLR4与NF-κB呈正相关(P<0.05),且与IL-10、IL-6和IL-17呈正相关(P<0.05),而与TGF-β_1呈负相关(P<0.05)。结论 Graves病患者血清TLR4、NF-κB、IL-10、IL-6和IL-17水平升高,而TGF-β_1水平降低,NF-κB信号通路激活和Treg/Th17平衡失调可能共同参与Graves病发病过程。Objective To investigate the concentration of Toll-like receptor 4 （TLR4）, nuclear factor κB （NF-κB） and regulatory T cells （Treg） / T helper cell 17 （Th17） related cytokines in patients with Graves＇ disease and their potential correlations. Methods A total of 62 patients initially diagnosed with Graves＇ disease and 45 healthy volunteers were involved in this study. The serum levels of thyroid stimulating hormone （TSH）, free triiodothyronine （FT3）, free tetraiodothyronine （FT4） and thyrotropin receptor antibody （TRAb） were measured by Electrochemiluminescence. The serum levels of TLR4, NF-κB, transforming growth factor β（TGF-β）, interleukin 10 （IL-10）, interleukin 6 （IL-6） and interleukin 17 （IL-17） were measured by enzyme-linked immunosorbent assay （ELISA）. Results The serum levels of TSH in patients were increased while FT3, FT4 and TRAb were decreased significantly when compared with those in volunteers （ P〈 0.05）. Blood concentration of TLR4, NF-κB, IL-10, IL-6 and IL-17 in patients was upregulated while concentration of TGF-β1 was downregulated in comparison with the healthy control group （ P〈 0.05）. Pearson correlation analysis showed that TSH was negatively correlated with TLR4, NF-κB, IL-10, IL-6 and IL-17 （ P〈0.05）, but positively correlated with TGF-β1 （ P〈0.05）. FT3 and FT4 were positively correlated with TLR4, NF-κB, IL-10, IL-6 and IL-17, but negatively correlated with TGF-β1 （ P〈0.05）. TLR4 was positively correlated with NF-κB, IL-10, IL-6 and IL-17 （ P〈0.05）, but negatively correlated with TGF-β1 （ P〈0.05）. Conclusions The serum levels of TLR4, NF-κB, IL-10, IL-6 and IL-17 in patients with Graves＇ disease are increased while level of TGF-β1 is decreased. Activation of NF-κB signaling pathway and Treg/Th17 imbalance might be involved in the pathogenesis of Graves＇ disease.

关 键 词：GRAVES病 Toll样受体4 核因子-ΚB 调节性T细胞/辅助性T细胞17 细胞因子 

分 类 号：R581.4[医药卫生—内分泌]