TESTIN在鼻咽癌中的表达及对鼻咽癌细胞系增殖、上皮-间质转化和侵袭转移的影响  

Expression of TESTIN in nasopharyngeal carcinoma and its effects on the proliferation,epithelial-mesenchymal transition,invasion and metastasis of nasopharyngeal carcinoma cell line in vitro

作　　者：刘新彦[1] 李建斌[2] 李志强[3] 王丽坤[1] 路继成 李晓丽[1] 刘晓燕[1] 

机构地区：[1]河北北方学院附属第二医院耳鼻咽喉头颈外科,河北省张家口市075100 [2]中国石油天然气集团公司中心医院耳鼻咽喉科 [3]河北北方学院附属第二医院病理科,河北省张家口市075100 [4]河北省张家口市宣化区人民医院检验科

出　　处：《河北医药》2017年第21期3205-3208,共4页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题计划项目(编号:20170181)

摘　　要：目的观察TESTIN在鼻咽癌组织和细胞中的表达,分析TESTIN过表达对鼻咽癌细胞增殖、上皮-间质转化(EMT)和侵袭转移的影响。方法采用免疫组化、Real time-PCR、Western-blot检测TESTIN在鼻咽癌组织和细胞系的表达状况。利用脂质体LipofectamineTM2000转染鼻咽癌5-8F细胞,Real time-PCR、Western-blot验证转染效率。MTT、细胞划痕实验、细胞侵袭实验检测TESTIN过表达对5-8F细胞增殖、EMT和侵袭转移的影响。Western-blot检测TESTIN过表达对侵袭转移相关基因表达的影响。结果 TESTIN在鼻咽癌组织的阳性表达率和表达水平明显低于正常鼻咽部组织,差异有统计学意义(P<0.05)。与人正常永生化鼻咽上皮细胞系NP69比较,鼻咽癌细胞系CNE1、CNE2、C666-1、5-8F、6-10B中TESTIN表达水平显著降低,差异有统计学意义(P<0.05)。转染pc DNA3.1/TESTIN的5-8F细胞中TESTIN表达显著上调(P<0.05)。TESTIN过表达显著抑制5-8F细胞增殖、EMT和侵袭转移,同时上调E-cadherin表达,下调N-cadherin、Vimentin、MMP-2、MMP-9表达(P<0.05)。结论鼻咽癌组织和细胞系中TESTIN表达显著降低,TESTIN过表达可抑制鼻咽癌5-8F细胞增殖、EMT和侵袭转移。Objective To observe the expression of TESTIN in nasopharyngeal carcinoma（ NPC）,and to explore the effects of TESTIN overexpression on the proliferation,epithelial-mesenchymal transition（ EMT）,invasion and metastasis of nasopharyngeal carcinoma cell line in vitro. Methods The expressions of TESTIN in NPC tissue specimens and nasopharyngeal carcinoma cell line were detected by immunohistochemical assay,Real time-PCR,Western-Blot,respectively.After transient transfection of TESTIN into NPC cell line 5-8 F by means of lipofectamine TM 2000,the expression of TESTIN was detected by Real time-PCR and Western-Blot. The effects of TESTIN overexpression on the proliferation,EMT,invasion and metastasis of nasopharyngeal carcinoma cells were detected by MTT,cell scratching assay and cell invasion assay,respectively. Moreover the effects of TESTIN overexpression on the expression of genes related with invasion and metastasis were detected by Western-Blot. Results The expression levels of TESTIN in NPC tissues were significantly lower than those in normal nasopharyngeal tissues（ P〈0. 05）. The expression levels of TESTIN in NPC cell line-CNE1,CNE2,C666-1,5-8 F and6-10 B were significantly decreased,as compared with those in human normal immortalized nasopharyngeal epithelial cell line NP69（ P〈0. 05）. However,the expression levels of TESTIN in 5-8 F cells transfected by pc DNA3. 1/TESTIN were significantly increased（ P〈0. 05）. The overexpression of TESTIN obviously inhibited the proliferation,EMT,invasion and metastasis of5-8 F cells,at the same time, which up-regulated the expressions of E-cadherin, however, which down-regulated the expressions of N-cadherin,Vimentin,MMP-2 and MMP-9（ P〈0. 05）. Conclusion The expression levels of TESTIN are significantly decreased in human NPC tissue and cell lines,moreover,TESTIN overexpression can inhibit the proliferation,EMT,invasion and metastasis of 5-8 F cells.

关 键 词：TESTIN 鼻咽癌 增殖 上皮-间质转化 侵袭转移 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]