康咳灵合剂中重楼皂苷Ⅰ、Ⅱ含量测定  

Content Determination of Polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture by HPLC

作　　者：彭敏[1] 石美琴[1] 童晓东[1] 杨水英[1] 许彦[1] 

机构地区：[1]南通市中医院,江苏南通226001

出　　处：《中国中医药信息杂志》2017年第11期75-77,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：南通市卫生和计划生育委员会科技项目(WQ2015065)

摘　　要：目的测定康咳灵合剂中重楼皂苷Ⅰ、Ⅱ的含量。方法采用HPLC,色谱柱为Poroshell 120 Ec-C18柱(4.6 mm×150 mm,4μm),以乙腈(A)-水(B)作为流动相进行梯度洗脱,检测波长为210 nm,流速为1 m L/min,柱温为30℃。结果重楼皂苷Ⅰ在1.009~10.09μg(r=0.999 6)范围内线性关系良好,平均回收率为97.31%(RSD=2.05%,n=6);重楼皂苷Ⅱ在0.640 5~6.405μg(r=0.999 8)范围内线性关系良好,平均回收率为96.41%(RSD=1.67%,n=6)。结论本方法简便、重复性好,结果准确,可用于测定康咳灵合剂中重楼皂苷的含量。Objective To establish a method for determining the contents of polyphyllin Ⅰ and polyphyllin Ⅱ in Kangkeling Mixture. Methods The contents of polyphyllin Ⅰ and Ⅱ were determined by HPLC gradient elution. Poroshell 120 Ec-C18 column（4.6 mm × 150 mm, 4 μm） was used; Acetonitrile-water（A∶B） was set as the mobile phase; the flow rate was 1.0 m L/min; the detection wavelength was 210 nm; column temperature was 30 ℃. Results Polyphyllin Ⅰ showed good linear relationship in the range of 1.009–10.09 μg（r=0.999 6）, and the average recovery was 97.31%（RSD=2.05%, n=6）. Polyphyllin Ⅱ showed good linear relationship in the range of 0.640 5– 6.405 μg（r=0.999 8）, and the average recovery was 96.41%（RSD=1.67%, n=6）. Conclusion The method is simple, with good repeatability and accurate results, which can be used to determine the contents of polyphyllin Ⅰ and Ⅱ in Kangkeling Mixture.

关 键 词：康咳灵合剂 高效液相色谱法 重楼皂苷Ⅰ 重楼皂苷Ⅱ 

分 类 号：R284.1[医药卫生—中药学]