银翘柴黄颗粒质量控制方法研究  

Study on Quality Control Method of Yinqiao Chaihuang Granules

作　　者：张丽先 王学方[2,3] 曹静亚[1,2] 李晓 李自红 李飞飞[1,2] 李智宁[1,2] 宁二娟 宋梦娇 魏悦[1,2] 

机构地区：[1]河南科高中标检测技术有限公司,河南郑州450000 [2]河南省生物技术开发中心,河南郑州450000 [3]河南省科高植物天然产物开发工程技术有限公司,河南郑州450002

出　　处：《中国中医药信息杂志》2017年第11期82-85,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河南省科技攻关项目(152102110115)

摘　　要：目的建立银翘柴黄颗粒质量控制方法。方法采用薄层色谱法鉴别银翘柴黄颗粒中金银花、柴胡、黄芩及甘草,HPLC测定连翘酯苷A的含量。结果在选定的色谱条件下,薄层色谱斑点清晰、分离度好,阴性对照未见干扰。含量测定连翘酯苷A在0.029 6~0.474μg范围内线性关系良好,平均回收率为99.73%,RSD=1.18%。结论本方法准确、简便,能够有效控制银翘柴黄颗粒的质量。Objective To establish the quality control method of Yinqiao Chaihuang Granules. Methods Lonicerae Japonicae Flos, Bupleuri Radix, Scutellariae Radix and Glycyrrhizae Radix et Rhizoma in Yinqiao Chaihuang Granules were identified by TLC, and the content of forsythoside A was detected by HPLC. Results Under the selected chromatographic conditions, TLC spots were clear, the separating was good, and non-interference was found in negative control. Forsythoside A in calibration curve showed good linearity in the range of 0.029 6–0.474 μg, with average recovery of 99.73%, RSD=1.18%. Conclusion The method is accurate and simple, which can be used for the quality control of Yinqiao Chaihuang Granules.

关 键 词：银翘柴黄颗粒 质量控制 薄层色谱法 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]