资阳市无偿献血者乙型肝炎病毒核酸筛查阳性人群特点研究  

Study of characteristics of HBV nucleic acid screening positive population among voluntary blood donors in Ziyang City

作　　者：王裕红 王华国[2] 

机构地区：[1]四川省资阳市中心血站质管科,641300 [2]四川省资阳市第一人民医院检验科,641301

出　　处：《检验医学与临床》2017年第20期3029-3031,共3页Laboratory Medicine and Clinic

摘　　要：目的研究无偿献血者乙型肝炎病毒(HBV)核酸筛查(NAT)阳性人群特点。方法选取该市2015年12月至2016年10月的无偿献血者20 000例,对其进行核酸混样定性检测、拆分单检及补充血清学检测,然后对其进行跟踪随访。结果 20000例无偿献血者中,HBV表面抗原(HBsAg)采用酶联免疫吸附试验法检测178例(0.890%)为HBsAg反应性,其中初次献血者的HBsAg反应性率明显高于重复献血者,差异有统计学意义(P<0.05)。HBsAg、抗丙型肝炎病毒抗体(-HCV)、抗-人类免疫缺陷病毒(HIV1/2)结果阴性18 000例,17例(0.094%)为混合检测(MPX)反应性,其中初次献血者和重复献血者的MPX反应率之间的差异无统计学意义(P>0.05)。17例MPX反应性标本中,11例(0.061%)为HBV DNA,其中初次献血者和重复献血者的HBV DNA比例之间的差异无统计学意义(P>0.05)。11例HBV DNA阳性标本中,10例做乙型肝炎补充试验,乙型肝炎补充血清学试验均为阴性2例,单纯抗-HBs阳性1例,抗-HBc阳性7例,其中单纯抗-HBc阳性6例,抗-HBs阳性1例。结论无偿献血者HBV核酸筛查能够使血液安全得到进一步保证。HBV核酸检测阳性献血者中抗-HBc阳性比例较高,为降低HBV经血传播的风险,建议未开展核酸检测的血站增加对血液检测抗-HBc项目。Objective To study the characteristics of hepatitis B virus（HBV）nucleic acid test（NAT）positive population in voluntary blood donors.Methods A total of 20 000 voluntary blood donors in the Ziyang Municipal Centralr Blood Stations from May2015 to May 2016 were selected and performed the nucleic acid mixed sample qualitative detection,splitand single detection,and supplementary serologic detection,then performed the follow up.Results Among 20 000 voluntary blood donors,178cases（0.890%）were HBsAg reactive by HBsAg ELISA detection,in which the HBsAg reactivity rate of the first time blood donors was significantly higher than that of the repeated donors,the difference was statistically significant（P〈0.05）.Among 18 000 cases of HBsAg anti-HCV and anti-HIV1/2results negative,17cases（0.094%）were MPX reactive,the MPX reactivity rate had no statistical difference between the first time blood donors and repeated donors（P〉0.05）.Among 17 cases of MPX reactive samples,11cases（0.061%）were HBV DNA,the HBV DNA ratio had no statistical difference between the first time blood donors and repeated donors（P〉0.05）.Among 11 cases of HBV DNA positive samples,10 cases conducted hepatitis B supplementary test and hepatitis B supplementary serological tests,2cases were negative,1case was simple anti-HBs positive,7cases were anti-HBc positive,among them 6cases were anti-HBc positive and 1case was anti-HBs positive.Conclusion Hepatitis B virus nucleic acid testing positive for anti-HBc positive ratio is higher in blood donors,in order to reduce the risk of blood transmission of hepatitis b virus,suggest not to carry out the nucleic acid detection of blood stations increase blood tests for detecting anti-HBc project.

关 键 词：无偿献血者 乙型肝炎病毒 核算筛查 

分 类 号：R512.62[医药卫生—内科学]