苦豆草总碱乳房灌注液对大鼠长期毒性研究  

Long-term toxicity test of total alkaloids from Sophora alopecuroides L. implantation in rats

作　　者：张虹[1,2] 梁剑平 刘宇[3] 郝宝成 尚若锋 郭文柱[3] 王学红 杨珍[3] ZHANG Hong LIANG Jianping LIU Yu HAO Baocheng SHANG Ruofeng GUO Wenzhu WANG Xuehong YANG Zhen(Gansu Agricultural University, Lanzhou Gansu 730070, China The Second Hospital of Gansu Province, Lanzhou Gansu 730000, China Key laboratory of New Animal Drug Project, Gansu Province, Key laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou institute of Husbandry and Pharmaceutieal sciences of CAAS, Lanzhou Gansu 730050, China)

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]甘肃省第二人民医院,甘肃兰州730000 [3]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中兽医医药杂志》2017年第5期45-47,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：基金项目:中国农业科学院科技创新工程兽用天然药物创新工程(CAAS-ASTIP-2014-LIHPS-04)

摘　　要：通过大鼠长期毒性试验研究评估苦豆草总碱乳房灌注液用于该疾病治疗的安全性。方法:采用计算机随机法将80只Wistar大鼠按雌雄随机平均分为4组:苦豆草总碱乳房灌注液高剂量组、中剂量组、低剂量组及对照组。各试验组经口灌胃分别给予不同剂量的苦豆草总碱乳房灌注液,对照组给予注射用纯净水,1次/d,共28 d。给药期间每天观察大鼠的行为表现,并测量体质量,干预结束后经尾脉采血1 m L,检测血液常规及生化等指标。结果表明,与对照组相比,不同剂量苦豆草总碱乳房注入剂干预组的大鼠体质量无明显异常变化,血液常规如白细胞、红细胞及血小板,生化指标如肝脏、肾脏功能等均无异常变化。同时,各重要脏器的大体解剖及组织学均未见明显病理变化。结果提示不同剂量的苦豆草总碱乳房灌注液对大鼠无明显的毒副作用。To study the toxicity and side effect of total alkaloids of Sophora alopecuroides L. implantation used on cow mastitis.Methods： 80 Wistar rats were randomly assigned into four groups,the high-dose group,middle-dose group,low-dose group,and control group. Rats were given different doses of the drug or placebo for 28 days once daily. The behavioral expression and body weight were measured every day. Finally,the blood routine and biochemical indexes were tested. Results： Compared with control group,behavioral expression,body weight,blood routine and biochemical indexes were not different in these experimental groups.Conclusion： The total alkaloids of Sophora alopecuroides L. implantation present no obvious toxicity and side effects on rats.

关 键 词：苦豆草总碱灌注液 长期毒性 大鼠 

分 类 号：S853.7[农业科学—临床兽医学]