经皮染毒反复束缚对SD大鼠生长的影响  

A study on the growth effects of SD rats by repeated restraint in dermal exposure test

作　　者：黄雅丽[1] 黎关龙[1] 来伟旗[1] 陈炜[1] 徐娟[1] 朱勇[1] 陈琼姜[1] 陈日萍[1] 张佳莉[1] 洪雅青[1] 张芳芳[1] 金锋[1] 顾刘金[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《预防医学》2017年第10期983-986,990,共5页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省科技厅科技条件建设项目(2013F10052)

摘　　要：目的分析经皮染毒反复束缚对大鼠生长的影响。方法 8周龄SD大鼠随机分为束缚组和对照组,每组20只,雌雄各半。束缚组大鼠模拟经皮染毒试验每天束缚固定6 h,连续91 d,对照组不作上述处理。观察两组大鼠临床症状、体重和摄食量变化,试验结束时进行血液学、血清生化和尿液生化检测,并作大体解剖和病理组织学检查,采用广义估计方程比较两组间差异。结果广义估计方程统计结果显示,无论雌雄大鼠,体重变化组别和试验时间存在交互作用(P<0.05),且束缚组雄性大鼠体重低于对照组(P<0.05)。进一步分析发现,雄性大鼠体重从试验第4周开始低于对照组(P<0.05),并且组别与试验时间间出现交互作用(P<0.05);雌性大鼠从试验第8周开始,组别与试验时间间出现交互作用(P<0.05)。束缚组雌性大鼠红细胞(RBC)、血红蛋白(HGB)、血容积比(HCT)和雄性大鼠RBC和HGB水平均高于对照组(P<0.05),但无生物学意义;两组间其他观察指标差异均无统计学意义(P>0.05)。结论经皮染毒反复束缚影响大鼠体重的增长,且雄性大鼠的敏感性高于雌性大鼠。Objective To analysis the effects on the growth of rats by repeated restraint in dermal exposure test. Methods SD rats in the restraint group was bound for 6 hours per day for 91 days according the way by dermal exposure, while SD rats in the control group didn＇t receive the treatment. Clinical signs, body weight and food consumption changes were observed for 91 days. When the study was terminated, hematology, clinical biochemistry, urinalyses, gross necropsy, and histopathology were carried out. Statistical methods such as the generalized estimating equation were used to compare the differences between two groups. Results The statistical results of generalized estimating equation showed that there was an interaction between the group and test time for male and female rats in body weight changes （P 〈 0.05） , and the body weight of male rats in the restraint group was lower than the control group （P 〈 0.05） . Further analysis showed that for male rats there was significant difference between groups since the forth week （P 〈 0.05） , and the interaction was found between groups and test time （P〈 0.05） . For female rats, the interaction was found since the eighth week between the group and test time （P 〈 0.05） .There was no significant differences in other parameters between two groups（P〉0.05） ‘ Conclusion Repeated restraint during dermal exposure affected the body weight gain of rats, and the sensitivity of male rats was higher than that of female rats.

关 键 词：经皮染毒 束缚 生长 广义估计方程 

分 类 号：R33[医药卫生—人体生理学]