慢性阻塞性肺疾病患者外周血核苷酸结合寡聚化结构域2、核苷酸结合寡聚化结构域样受体蛋白3及下游炎性因子表达情况及其关系研究  

Peripheral Blood Expressions of NOD2,NLRP3 and Downstream Inflammatory Cytokines in Patients with COPD and Their Correlations

作　　者：宋珊[1] 贾钦尧 陈绍平[1] 毛建[1] 韩美玲[1] 廖俊蕾[1] 

机构地区：[1]川北医学院附属医院呼吸内科,四川省南充市637000

出　　处：《实用心脑肺血管病杂志》2017年第9期42-46,共5页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：四川省教育厅基金资助项目(16ZB0230)

摘　　要：目的探讨慢性阻塞性肺疾病(COPD)患者外周血核苷酸结合寡聚化结构域2(NOD2)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)及下游炎性因子表达情况及其关系。方法选取2015年1月—2017年1月川北医学院附属医院收治的COPD患者200例,其中慢性阻塞性肺疾病急性发作期(AECOPD)患者100例作为A组,COPD缓解期患者100例作为B组,并根据肺功能分级及1年内急性发作次数将B组患者分为低风险组与高风险组,每组50例;另选取同期体检健康者100例作为对照组。比较A组、B组、对照组和低风险组、高风险组、对照组受试者NOD2 mRNA表达量、NLRP3 mRNA表达量、血清白介素1β(IL-1β)和白介素18(IL-18)水平,NOD2 mRNA表达量、NLRP3 mRNA表达量与COPD患者血清IL-1β水平、血清IL-18水平、第1秒用力呼气容积与用力肺活量比值(FEV_1/FVC)、第1秒用力呼气容积占预计值百分比(FEV_1%)的相关性分析采用Pearson相关性分析。结果 A组、B组患者NOD2 mRNA表达量、NLRP3 mRNA表达量、血清IL-1β和IL-18水平高于对照组(P<0.05);A组患者NOD2 mRNA表达量、NLRP3 mRNA表达量、血清IL-1β和IL-18水平高于B组(P<0.05)。低风险组、高风险组患者NOD2 mRNA表达量、NLRP3 mRNA表达量、血清IL-1β和IL-18水平高于对照组(P<0.05);高风险组患者NOD2 mRNA表达量、NLRP3 mRNA表达量、血清IL-1β和IL-18水平高于低风险组(P<0.05)。Pearson相关性分析结果显示,NOD2 mRNA表达量、NLRP3 mRNA表达量与COPD患者血清IL-1β水平(r值分别为0.625、0.656)、血清IL-18水平(r值分别为0.701、0.723)呈正相关,与FEV_1/FVC(r值分别为-0.389、-0.415)、FEV_1%(r值分别为-0.513、-0.558)呈负相关(P<0.05)。结论 COPD患者外周血NOD2、NLRP3表达水平及血清IL-1β、IL-18水平较高,且NOD2 mRNA表达量、NLRP3 mRNA表达量与COPD患者血清IL-1β、IL-18水平及肺功能有关。Objective To investigate the peripheral blood expressions of NOD2, NLRP3 and downstream inflammatory cytokines in patients with COPD and their correlations. Methods A total of 200 patients with COPD were selected in the Affiliated Hospital of North Sichuan Medical College from January 2015 to January 2017,thereinto 100 patients with acute exacerbation of COPD were served as A group,other 100 patients with stable COPD were served as B group,and then patients of B group were divided into low-risk group and high-risk group according to the pulmonary function grading and acute exacerbation frequency within 1 year,each of 50 cases; meanwhile 100 healthy people admitted to this hospital for physical examination were selected as control group. Expressions of NOD2 mRNA and NLRP3 mRNA,serum levels of IL-1β and IL-18 were compared among A group,B group and control group,among low-risk group,high-risk group and control group;Pearson correlation analysis was used to analyze the correlations of expressions of NOD2 mRNA and NLRP3 mRNA with serum levels of IL-1β and IL-18,FEV_1/FVC and FEV_1% in patients with COPD. Results Expressions of NOD2 mRNA and NLRP3 mRNA,serum levels of IL-1β and IL-18 of A group and B group were statistically significantly higher than those of control group,meanwhile expressions of NOD2 mRNA and NLRP3 mRNA, serum levels of IL-1β and IL-18 of A group were statistically significantly higher than those of B group（ P 0. 05）. Expressions of NOD2 mRNA and NLRP3 mRNA,serum levels of IL-1β and IL-18 of low-risk group and high-risk group were statistically significantly higher than those of control group,meanwhile expressions of NOD2 mRNA and NLRP3 mRNA, serum levels of IL-1β and IL-18 of high-risk group were statistically significantly higher than those of low-risk group（ P 0. 05）. Pearson correlation analysis results showed that,NOD2 mRNA expression and NLRP3 mRNA expression was positively correlated with serum levels of IL-1β（ r = 0. 625; r = 0. 656） and IL-18（ r = 0. 701; r = 0. 723�

关 键 词：肺疾病 慢性阻塞性 核苷酸结合寡聚化结构域2 核苷酸结合寡聚化结构域样受体蛋白3 炎性因子 

分 类 号：R563.9[医药卫生—呼吸系统]