SAMHD1对HBV DNA形成的抑制作用及其机制研究  

Inhibitory effect of SAMHD1 on production of HBV DNA

作　　者：程璐[1] 王青青[1] 

机构地区：[1]浙江大学基础医学院免疫学研究所,杭州310058

出　　处：《中国免疫学杂志》2017年第10期1474-1477,共4页Chinese Journal of Immunology

摘　　要：目的:研究不同肝细胞SAMHD1表达水平及其对HBV DNA形成的抑制作用和相关机制。方法:通过实时荧光定量PCR和Western blot法检测Jurkat、THP-1、Hep G2、Hep G2.2.15和Huh7细胞等细胞SAMHD1 mRNA和蛋白表达水平;通过对Hep G2.2.15细胞转染过表达质粒和shRNA,升高或者降低SAMHD1的表达水平,观察不同SAMHD1表达水平对HBV DNA水平的影响;补充不同浓度的d NTP,比较不同d NTP浓度对HBV DNA水平的影响。结果:肝癌细胞系有较高的SAMHD1表达水平;降低SAMHD1可以使HBV DNA水平升高2.3~2.8倍,升高SAMHD1可以使HBV DNA降低至33%左右(P<0.01);升高d NTP浓度可以促进HBV DNA的合成水平,抵消SAMHD1的抑制作用。结论:SAMHD1可以抑制肝癌细胞内HBV DNA的生成,可能是通过降低细胞内d NTP浓度实现的。Objective： To study the expression levels of SAMHD1 in different liver cancer cells and the inhibition of HBV DNA production by SAMHD1. Methods： SAMHD1 mRNA and protein levels in Jurkat,THP-1,Hep G2,Hep G2. 2. 15 and Huh7 were detected by Real-time quantitative PCR and Western blot. SAMHD1 expression in Hep G2. 2. 15 was increased by transfecting SAMHD1 over expression vectors or was inhibited by transfecting SAMHD1 specific shRNA,and then HBV DNA levels were detected. The effect of different d NTP concentration on HBV DNA production was detected also. Results： SAMHD1 were highly expressed in liver cancer cells. Decreased SAMHD1 expression could enhance HBV DNA production by 2. 3-2. 8 times,and increased SAMHD1 expression could inhibit HBV DNA levels by 3 times（ P〈0. 01）. Increased d NTP concentration could enhance HBV DNA production and counteract the inhibitory effect of SAMHD1. Conclusion： SAMHD1 can inhibit the production of HBV DNA in liver cancer cells by decreasing d NTP concentration.

关 键 词：SAMHD1 HBV 肝癌细胞 DNTP DNA 宿主限制因子 

分 类 号：R392.11[医药卫生—免疫学]