MIC基因多态性与解脲脲原体感染的易感关联性研究  

Study on association between MIC gene polymorphism and ureaplasma urealyticum infection

作　　者：李瑜 陈恩 陈纯静[3] 曾凡胜 周琳 曾威 

机构地区：[1]益阳医学高等专科学校,益阳413000 [2]南华大学附属第一医院,衡阳421001 [3]长沙医学院,长沙410219 [4]益阳市中心医院,益阳413000

出　　处：《中国免疫学杂志》2017年第10期1478-1482,共5页Chinese Journal of Immunology

摘　　要：目的:研究MIC等位基因多态性与解脲脲原体感染易感性之间的关联性。方法:采用PCR-SSP和PCR-SBT方法对样本MIC等位基因的多态性进行检测。结果:解脲脲原体患者中检出12种MICA、5种MICA-STR和14种MICB等位基因;和对照组相比较,解脲脲原体患者组中MICA*010和MICB*009N等位基因分布频率更高(MICA*010:OR=3.85,95%CI:2.12-6.99,Pc<0.05;MICB*009N:OR=3.22,95%CI:1.33-7.80,Pc<0.05),MICA-A5.1和MICB*00502等位基因分布频率更低(MICA-A5.1:OR=0.61,95%CI:0.40-0.94,Pc<0.05;MICB*00502:OR=0.58,95%CI:0.40-0.83,Pc<0.05),差异均具有统计学意义;基因型方面,MICA*010/010、MICA*01201/01201纯合子分布频率更高(MICA*010/010:OR=14.84,95%CI:1.90-115.9,Pc<0.05;MICA*01201/01201:OR=10.83,95%CI:1.35-86.79,Pc<0.05),差异均具有统计学意义;解脲脲原体患者中MICB*00502/00502纯合子分布频率较高,但差异不具有统计学意义(Pc>0.05)。结论:MIC等位基因多态性与解脲脲原体的易感性间存在关联性。Objective： To investigate the association between MIC allele polymorphism and susceptibility to Ureaplasma urealyticum infection. Methods： PCR-SSP and PCR-SBT were used to analyze the gene polymorphism of every one. Results： Twelve allele genes of MICA and five MICA-STR and fourteen MICB were found in the participants,the frequency of MICA＊ 010 and MICB＊009N were higher in ureaplasma urealyticum infection patients than in healthy controls（ MICA＊ 010： OR = 3. 85,95% CI： 2. 12-6. 99,Pc〈0. 05; MICB＊ 009N： OR = 3. 22,95% CI： 1. 33-7. 80,Pc〈0. 05）; the frequencies of MICA-A5. 1 and MICB＊ 00502 were lower in ureaplasma urealyticum infection patients than in healthy controls（ MICA-A5. 1： OR = 0. 61,95% CI： 0. 40-0. 94,Pc〈0. 05; MICB ＊00502： OR = 0. 58,95% CI： 0. 40-0. 83,Pc〈0. 05）,the differences were statistically significant; MICA ＊ 010/010,MICA ＊ 01201/01201 homozygote were higher in ureaplasma urealyticum infection patients than in healthy controls（ MICA ＊ 010/010： OR = 14. 84,95% CI： 1. 90-115. 9,Pc〈0. 05： MICA＊ 01201/01201： OR = 10. 83,95% CI： 1. 35-86. 79,Pc〈0. 05）,the differences were statistically significant. The distribution frequency of MICB＊ 00502/00502 in patients with ureaplasma urealyticum was higher,but the difference was not statistically significant（ Pc〈0. 05）. Conclusion： MIC allele gene polymorphism may be associated with ureaplasma urealyticum infection.

关 键 词：MIC基因 基因多态性 解脲脲原体 

分 类 号：R392[医药卫生—免疫学]