两性霉素B前体脂质体的制备工艺研究  

Preparation Technology of Amphotericin B Proliposomes

作　　者：隋琳[1,2] 田野 王永利[2] 王立华[2] 庚丽丽[2] 赵瑶 王璐璐 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010100 [2]河北北方学院应用化学研究所,河北张家口075000 [3]河北北方学院理学院,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2017年第1期29-32,共4页Journal of Hebei North University:Natural Science Edition

基　　金：河北省科学技术研究与发展计划项目(12276104D-94);河北省高等学校科学研究计划项目(QN2015221);河北省高等学校科学技术研究青年基金项目(QN2015148)

摘　　要：目的研究两性霉素B前体脂质体制备工艺。方法采用载体沉积法制备两性霉素B前体脂质体,以包封率为评价指标,采用单因素试验和正交试验优化处方,并观察其重现性和稳定性。结果两性霉素B的最优处方工艺条件是膜材比为1∶2、载脂比为5∶1、药脂比1∶8。载体为甘露醇,最优处方下平均包封率(63.4±1.5)%。结论由最佳处方工艺条件制备的两性霉素B前体脂质体的包封率和稳定性高,重现性好。Objective To study preparation technology of amphotericin B proliposomes.MethodsAmphotericin B proliposomes were prepared by carrier aggradation method.A single-factor investigation and orthogonal design were used to select the optimum formulation.And the repeatability and stability were observed.Results The best prescription of chrysophanol proliposomes was as follows:the ratio of cholesterol to phospholipid was 1∶2,the ratio of carrier to phospholipid was 5∶1,and the ratio of drug to phospholipid was 1∶8.The best carrier was mannnitol.The average encapsulation efficiency of the optimal prescription was:(63.4±1.5)%.Conclusion By using the best prescription amphotericin B was highly entrapped into proliposomes with good reproducibility and stability.

关 键 词：两性霉素B 前体脂质体 载体沉积法 低速离心法 包封率 

分 类 号：R943[医药卫生—药剂学]