罗丹明甲醛酰肼类Cu^(2+)和Hg^(2+)双识别荧光探针  

Recognition of Cu^(2+) and Hg^(2+) by a Rhodamine Formaldehyde Acylhydrazine Based Dual Channel Fluorescence Probe

作　　者：袁跃华[1] 朱永军[1] 王海雁[1] 田茂忠[1] 冯锋[1] 

机构地区：[1]山西大同大学化学与环境工程学院,山西大同037009

出　　处：《山西大同大学学报（自然科学版）》2017年第5期34-37,共4页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：国家自然科学基金项目[21175085;21375083];大同市基础研究计划项目[2017126]

摘　　要：设计合成了一种对Cu^(2+)和Hg^(2+)具有双识别功能的荧光增强型分子探针3',6'-双(二乙氨基)-2-(亚甲氨基)螺[异吲哚-1,9'-氧杂蒽]-3-酮,并用核磁和质谱对其结构进行了表征,同时对目标荧光探针进行了荧光光谱性能测试。研究表明:探针溶液本身荧光很弱,在乙醇/水(V/V,1∶1)体系中与Hg^(2+)作用后荧光显著增强,但在乙腈/水V/V,1∶1)体系中Cu^(2+)会诱导其荧光显著增强,由此建立了基于一种探针对Cu^(2+)和Hg^(2+)测定的新方法。Cu^(2+)浓度在1.83×10^(-7)~3.58×10^(-6)mol/L范围内,Hg^(2+)浓度在1.18×10^(-7)~5.05×10^(-6)mol/L范围内,探针的荧光强度均与之呈现良好的线性关系。In this paper, a fluorescence enhancement probe,3＇,6＇-bis（diethylamino）-2-（methyleneamino）spiro[isoindoline-1,9＇-xanthen]-3-one（RNM）, for the recognition of Cu^2＋and Hg^2＋has been synthesized. Its structure was characterized by NMR and mass spectrometry. The fluorescence properties of the probe was tested by fluorescence spectra. The results showed that the fluorescence intensity of the free probe in the solution is very weak. However, Hg^2＋induced fluorescence significantly enhancement in ethanol/water（V/V, 1 ： 1） solution. Cu^2＋ irritated the fluorescence enhancement in acetonitrile/water（V/V, 1 ： 1）. Thus a new method for the detection of Cu^2＋and Hg^2＋by a rhodamine-based probe was established. The fluorescence intensity of the probe showed a good linear relationship with the concentration of Cu^2＋（from 1.83×10^-7 to 3.58×10^-6mol/L） and the concentration of Hg^2＋（from 1.18×10^-7 to 5.05×10^-6mol/L）.

关 键 词：罗丹明 CU^2+ HG^2+ 荧光增强型探针 

分 类 号：O657.34[理学—分析化学]