禽波氏杆菌HagA蛋白基因的序列分析及抗原表位预测  

Sequencing and B-cell epitopes prediction of Bordetella avium HagA protein

作　　者：袁朋[1] 朱瑞良[1] 崔晨晨 杨萍萍[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《浙江农业学报》2017年第10期1642-1647,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31602067;31272595);山东省自然科学基金项目(ZR2014CM044);山东省"双一流"奖补资金

摘　　要：试验旨在对禽波氏杆菌的血凝素HagA蛋白的二级结构与B细胞抗原优势表位进行预测,探讨以血凝素HagA蛋白制作单克隆抗体的可能性。首先对禽波氏杆菌的血凝素hagA基因进行了PCR扩增以及序列测定,利用生物信息学的相关软件与分析方法,对血凝素HagA蛋白的二级结构以及B细胞抗原表位进行分析预测。经综合分析表明,禽波氏杆菌的HagA蛋白氨基酸序列中存在7个B细胞优势抗原表位区域,6个可能的糖基化位点。研究结果为进一步分析禽波氏杆菌HagA蛋白的生物学功能、制备单抗和研制疫苗等奠定了基础。This article is carried out to predict the secondary structure of Bordetella avium HagA and the dominant epitope of B cell antigen,in order to discuss the possibility of HagA protein in the production of monoclonal antibodies. In this study,hag A gene of B. avium was amplified,cloned and sequenced. The secondary structure and B cell epitopes of the HagA protein of B. avium were analyzed and predicted subsequently by using bioinformatics software.The results showed that the B. avium HagA protein was supposed to include 7 potential antigen epitopes and 6 potential glycosylated sites. The results provide a theoretical basis for the further study of immune mechanisms,monoclonal antibodies preparation and epitope vaccine design.

关 键 词：禽波氏杆菌 HagA蛋白 二级结构 B细胞抗原表位预测 

分 类 号：S858.3[农业科学—临床兽医学]