离子液体预处理对马铃薯茎叶厌氧消化的影响  

Effect of Ionic Liquids Pretreatment on Anaerobic Digestion of Potato Stem Leaves

作　　者：葛一洪[1,2] 邱凌[2,3] 罗时海 李姗珊[2,3] 于秀男 郭晓慧[2,3] GE Yihong QIU Ling LUO Shihai LI Shanshan YU Xiunan GUO Xiaohui(College of Agronomy, Northwest A＆F University, Yangling, Shaanxi 712100, China Western Scientific Observing and Experimental Station for Development and Utiliation of Rural Renewable Energy, Ministry of Agriculture, Northwest A ＆F University, Yangling, Shaanxi 712100, China College of Mechanical and Electronic Engineering, Northwest A＆F University, Yangling, Shaanxi 712100, China)

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]西北农林科技大学农业部农村可再生能源开发利用西部科学观测实验站,陕西杨凌712100 [3]西北农林科技大学机械与电子工程学院,陕西杨凌712100

出　　处：《农业机械学报》2017年第10期266-271,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金面上项目(51576167)

摘　　要：马铃薯茎叶是一种丰富的木质纤维素生物质资源,但其高结晶度、稳定的刚性结构使其不易水解酸化,微生物在短时间内难以直接高效利用。研究了离子液体和助溶剂对马铃薯茎叶进行预处理后其木质纤维素结构组分变化及厌氧消化产沼气的变化。结果表明,预处理后的马铃薯茎叶木质素质量分数降低31.8%~43.9%,离子液体脱除马铃薯茎叶木质素能力由高到低依次为[C_2mim]Ac、[C_2mim]Ac/DMSO、[C_4mim]Cl/DMSO、DMSO、[C_4mim]Cl。离子液体中阴离子可与纤维素羟基上的氢质子形成氢键作用,降低其结晶度,长链分子断裂成短链分子,使厌氧消化的启动滞后期较未处理马铃薯茎叶提前9 d,累积产沼气量提高3.2%~76.3%,最高产甲烷体积分数提高14.3%~25.1%。各处理组累积产沼气量和最高产甲烷体积分数分别为:T5(7.21 L,69.2%)、T4(5.12 L,64.5%)、T2(5.07 L,65.7%)、T3(4.35 L,64.3%)、T1(4.22 L,63.2%)。经无机盐-离子液体双水相体系回收DMSO、[C_2mim]Ac和[C_4mim]Cl,回收率分别为95.1%、91.8%和89.6%。With the fulfillment of China＇s potato staple food strategy, potato stem leaves gradually become plentiful lignocellulosic biomass resources. However, its cell wall has high-crystalline structure and available surface area which were difficult to hydrolyze and produce biogas by microorganism. Therefore, the effect of lignocellulosic composition, structural and anaerobic digestion from the pretreated potato stem leaf by ionic liquids and dimethyl sulfoxide was evaluated. The potato stem leaf was pretreated with ionic liquids l-N-butyl-3-methyimidazolium chloride （ [ C4 mim] Cl ） , 1-ethyl-3-methlyimidazolium acetate （[ C2mim] Ae） and dimethyl sulfoxide （DMSO） under the condition of 130℃ for 120 min. Compared with untreated group （19.8% lignin content） , the composition and structure of lignocellulosic from the regenerated potato stein leaf was changed and the lignin content was reduced by 31.8% -43.9%. The dissolving capability of the solvents followed the order： [ C2mim] Ac, [ C2mim] Ac/DMSO, [ C4mim] Cl/ DMSO, DMSO, [ C4 mim ] Cl. The anaerobic digestion data demonstrated the lag phase of anaerobic digestion start-up period was shortened by 9 d. Because the solubility of cellulose was risen with the increase of the H-bonds accepting ability of the anions which would form more efficient H-bonds between the hydroxyl protons of cellulose and the anion, meanwhile, the crystalline structure of cellulose became lower and the surface became more porous. In other words, the hydrolytic acidification stage was almost accomplished after pretreatment. Plenty of the small molecular substances, which were easy to be used by methanogens, were dissolved out. The cumulative biogas production and the highest methane content were increased by 3.2% - 76.3% and 14.3% - 25. stem leaf. The cumulative biogas production and the 1%, respectively, compared with untreated potato highest methane content of each pretreated groups were 7.21Land69.2% （[C4mim]Cl/DMSO）, 5.12 L and 64.5% （[C2mim]Ac/DMSO）, 5.07 L and

关 键 词：马铃薯茎叶 离子液体 木质纤维素 厌氧消化 

分 类 号：X71[环境科学与工程—环境工程] S216.4[农业科学—农业机械化工程]