蛋白质在膜表面的吸附特性及蛋白质存在的溶液环境对小檗碱膜透过行为的影响研究  

Adsorption characteristics of proteins on membrane surface and effect of protein solution environment on permeation behavior of berberine

作　　者：李益群 徐丽[1] 朱华旭[1] 唐志书 李博[1] 潘永兰[1] 姚薇薇[1] 付廷明[1] 郭立玮[1,2] LI Yi-qun XU Li ZHU Hua-xu TANG Zhi-shu LI Bo PAN Yong-lan YAO Wei-wei FU Ting-ming GUO Li-wei(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712046, China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]陕西中医药大学陕西省中药资源产业化协同创新中心,陕西咸阳712046

出　　处：《中国中药杂志》2017年第20期3912-3918,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81673610;81773912);国家"重大新药创制"科技重大专项(2011ZX09401-308-037);江苏省科技厅重点研发计划--社会发展面上项目(BE2016754);江苏省六大人才高峰项目(2014-YY-014);江苏省"青蓝工程"项目

摘　　要：为探索蛋白质在膜表面的吸附特性以及蛋白质存在的溶液环境对小檗碱膜透过行为的影响,以蛋白质、小檗碱为研究对象,配制模拟溶液,通过低场核磁共振技术、静态吸附实验以及膜分离实验探究蛋白质与陶瓷膜、小檗碱之间可能存在的相互作用,以蛋白质静态吸附量、膜过程相对通量、蛋白质截留率、小檗碱透过率及两者的吸附率为评价指标,同时测定膜过程中膜污染阻力分布、过膜前后粒径分布变化及膜表面电镜扫描(SEM),研究蛋白质在陶瓷膜上的吸附特性及对小檗碱膜过程的影响。结果表明,陶瓷膜对蛋白质具有吸附作用,且吸附模型符合Langmuir吸附模型,在模拟溶液的膜分离过程中,蛋白质是引起膜污染的主要因素,但在1 g·L^(-1)蛋白质的浓度下,蛋白质的存在对小檗碱的膜过程没有显著影响。In order to explore the adsorption characteristics of proteins on the membrane surface and the effect of protein solution environment on the permeation behavior of berberine, berberine and proteins were used as the research object to prepare simulated solu- tion. Low field NMR, static adsorption experiment and membrane separation experiment were used to study the interaction between the proteins and ceramic membrane or between the proteins and berberine. The static adsorption capacity of proteins, membrane relative flux, rejection rate of proteins, transmittance rate of berberine and the adsorption rate of proteins and berberine were used as the evalu- ation index. Meanwhile, the membrane resistance distribution, the particle size distribution and the scanning electron microscope （SEM） were determined to investigate the adsorption characteristics of proteins on ceramic membrane and the effect on membrane sepa- ration process of berberine. The results showed that the ceramic membrane could adsorb the proteins and the adsorption model was con- sistent with Langmuir adsorption model. In simulating the membrane separation process, proteins were the main factor to cause mem-brane fouling. However, when the concentration of proteins was 1 g · L^-1, the proteins had no significant effect on membrane separa- tion process of berberine.

关 键 词：蛋白质 小檗碱 陶瓷膜微滤 模拟体系 吸附特性 

分 类 号：R284[医药卫生—中药学]