维药阿纳其根抗肿瘤活性部位筛选及其化学成分的分离鉴定  

Study on screening antitumor active fractions and chemical components in active fractions from root of Anaycclus pyrethrum

作　　者：董洁[1] 袁将 王加利[1] 姬瑞芳 全庆华 郭晓宇 高简 刘永刚[1] DONG Jie YUAN Jiang WANG Jia-li JI Rui-fang QUAN Qing-hua GUO Xiao-yu GAO Jian L(School of Chinese Materia Medica, Belting University of Chinese Medicine, Beijing 100102, China)

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2017年第20期3932-3937,共6页China Journal of Chinese Materia Medica

摘　　要：为探寻阿纳其根的生物活性成分,对其抗肿瘤活性部位化学成分进行了研究。结果表明,主要抗肿瘤活性部位为二氯甲烷部位和乙酸乙酯部位。从阿纳其根的二氯甲烷部位和乙酸乙酯部位共分离得到10个单体成分,其中,包括7个N-烷基酰胺类生物碱、1个香豆素和2个黄酮苷。分别为十四-2E,4E,8E-三烯酸-4-羟基苯乙胺(1)、十-2E,4E-二烯酸异丁基胺(2)、十一-2E,4E-二烯-8,10二炔酸苯乙胺(3)、十四-2E,4E-二烯酸-4-羟基苯乙胺(4)、十四-2E,4E-二烯-8,10-二炔-异丁基胺(5)、十-2E,4E-二烯酸苯乙胺(6)、十二-2E,4E-二烯酸苯乙胺(7)、异莨菪亭(8)、槲皮素-7-O-β-D-吡喃葡萄糖苷(9)、异鼠李素-7-O-β-D-吡喃葡萄糖苷(10)。活性部位分离得到的化合物1为1个新的化合物,化合物2~4,化合物8~10为首次从该植物中分离得到。Components that systematic separated from the root of Anaycclus pyrethrum were identified, in order to lay a foundation for future study of the root of A. pyrethrum. The CCK-8 assay showed that dichloromethane fraction exhibited the highest degree of cyto- toxicity than others. Ten monomeric components were obtained from dichloromethane fraction and ethyl acetate fraction extracted from the root of A. pyrethrum, including 7 N-alkylamides, one coumarin and two flavonoid glycosides. They were identified as tetradeca-2E, 4E,8E-trienoic acid 4-hydroxyphenylethylamide（1）, deca-2E,4E-dienoicacid isobutylamide（2）, undeca-2E,4E-diene-8,10-diynoic acid phenylethylamide （ 3 ）, tetradeca-2E, 4E-dienoic acid 4-hydroxyphenylethylamide （ 4 ）, tetradeca-2E, 4E-diene-8,10-diynoic acid isobutylamide （5）, deca-2E,4E- dienoic acid 4-hydroxyphenylethylamide （6）, dodeca-2E,4E-dienoic acid 4-hydroxy -phenyl-ethylam- ide （ 7 ）, isoscopoletin （ 8 ） , quercetin-7-O-β-D-glucopyranoside （ 9 ）, isorhamnetin-7-O-β-D-glucopyranoside （ 10 ）. Among them, com- pound 1 was identified as a new compound. Compounds 2-4. 8-10 were isolated from this herb for the first time.

关 键 词：阿纳其根 抗肿瘤活性 化学成分 N-烷基酰胺类生物碱 香豆素 黄酮苷 

分 类 号：R29[医药卫生—民族医学]