鲤IL-10锦鲤疱疹病毒核酸疫苗联合免疫效果的研究  

Immune effect of carp Interleukin-10 and Koi Herpesvirus nucleic acid vaccine combined immunization

作　　者：于慧[1] 王好[1] 祖岫杰[2] 康学会 杨炳坤 刘艳辉[2] 周井祥[1] YU Hui WANG Hao ZU Xiu-jie KANG Xue-hui YANG Bing-kun LIU Yan-hui ZHOU Jing-xiang(Institute of Animal Science and Technology of Jilin Agricultural University, Changchun 130118, China Jilin Academy of Fishery Sciences, Changchun 130033, China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林省水产科学研究院,吉林长春130033

出　　处：《中国兽医杂志》2017年第8期83-85,90,共4页Chinese Journal of Veterinary Medicine

基　　金：国家现代农业产业技术体系建设项目(CARS 4629);吉林省自然科学基金项目(20140101035JC)

摘　　要：本研究选用鲤IL-10,构建p EGFP-N1-IL-10真核表达质粒,分别进行体外细胞转染试验和免疫鱼的试验,证明了重组质粒在细胞中能成功表达,体内免疫试验ELISA结果显示,抗体水平会随着免疫次数、剂量的增加逐渐升高,三免后第1周的抗体水平最高,随后抗体水平逐渐下降。相比单一核酸疫苗p IRES-ORF81,p EGFP-N1-IL-10与核酸疫苗p IRES-ORF81联合免疫所产生抗体水平明显升高,但差异不显著(P>0.05)。The eukaryotic recombinant plasmid p EGFP-N1-IL-10 were constructed based on IL-10 m RNA. In vitro cell transfec-tion experiment, proved that the recombinant plasmid was successfully expressed in cells. ELISA results showed that antibody levelsincreased gradually with the increase of number and dose of immunization, The highest antibody level was on the first week after threeimmune. Then the antibody levels gradually declined. Compared with the single nucleic acid vaccine p IRES-ORF81, antibody levelsin fish immunized with the combination of p EGFP-N1-IL-10 and nucleic acid vaccine p IRES-ORF81 increased, but it was not signif-icant（P〉0. 05）.

关 键 词：锦鲤疱疹病毒 鲤白细胞介素10 核酸疫苗p IRES-ORF81 

分 类 号：S942.5[农业科学—水产养殖]