量子点-罗丹明B比率型荧光探针的制备与性能  

Preparation and properties of QDs and RhB ratiometric fluorescent probe

作　　者：梁潇[1] 袁野[1] 曹婷婷[1] 董丽杰[1] Xiao Liang Ye Yuan Tingting Cao Lijie Dong(Center for Smart Materials and Device Integration, Wuhan University of Technology, Wuhan 430070, Chin)

机构地区：[1]武汉理工大学智能材料与器件研究中心,武汉430070

出　　处：《中国科学：化学》2017年第10期1191-1197,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:51273157);湖北省自然科学基金(编号:2016CF008)资助项目

摘　　要：本文采用CdSe/Cd_xZn_(1-x)S核/合金壳量子点(QDs)作为荧光共振能量转移体系(FRET)的供体,通过巯基络合作用在其表面修饰一层L-半胱氨酸(Cys)分子,赋予QDs优异的水溶性能,再通过静电相互作用将罗丹明B(RhB)构筑于QDs-Cys表面,获得了一类新型的水溶性FRET体系.采用荧光光谱分析了pH以及供受体浓度比对FRET能量转移效率的影响.研究结果表明,通过静电结合构筑的QDs-Cys-RhB荧光共振能量转移体系对pH和供受体浓度具有敏感的荧光信号响应性能.当pH从10降到7时,FRET体系的荧光共振能量转移效率由49.39%增加到58.99%;当供受体浓度比为3:1时,FRET体系的能量转移效率高达61.09%.由此可见,通过表面络合与静电相互作用构筑的QDs-Cys-RhB荧光共振能量转移体系具有优异的光信号响应性,可以作为一类灵敏、精确的可调式比率型荧光探针,在生物检测、免疫分子等领域中具有广阔的应用前景.Due to the unique quantum size effect, surface effect and macro quantum tunnel effect, QDs have attracted much attention in the past decade as a new class of biological nanoprobe. In this article, the multicomponent alloyed quantum dots were modified by L-cysteine to form aqueous QDs which showed good fluorescence properties. The FRET system was built by linking the energy donor QDs with Rhodamine B as acceptor. Their photoluminescence ability was further characterized. The result indicated that the energy transfer efficiency increased with pH 10 to 7. Besides, The FRET efficiency increased with the donor/acceptor concentration ratio within a certain scope, can be as high as 61.09%when the ratio was 3：1. Therefore, the as-constructed FRET system via positive and negative ions reaction exhibited a well-resolved response at pH values and donor/acceptor concentration ratio. Such QDs-Cys-RhB FRET system has a great potential as an efficient and sensitive ratiometric fluorescence nanoprobe in biological application.

关 键 词：荧光共振能量转移 量子点 罗丹明B 可调式比率型荧光探针 

分 类 号：O657.3[理学—分析化学]