Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing  

作　　者：Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University [2]Section of Cell and Developmental Biology,University of California San Diego

出　　处：《Journal of Genetics and Genomics》2017年第9期469-472,共4页遗传学报（英文版）

基　　金：supported by a National Transgenic Science and Technology Program (2016ZX08010002)to R.W.;a startup fund from the Huazhong Agricultural University

摘　　要：Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks（DSBs）in vivo is a prerequisite for any genome editing efforts.Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks（DSBs）in vivo is a prerequisite for any genome editing efforts.

关 键 词：RGR Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing 

分 类 号：Q943.2[生物学—植物学]