Effects of difructose dianhydride (DFA)-Ⅳ on in vitro fertilization in pigs  

作　　者：Young-Joo Yi S. Kamala-Kannan Jeong-Muk Lim Byung-Taek Oh Sang-Myeong Lee 

机构地区：[1]Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 54596, Korea [2]Safety, Environment and Life Science Institute, Chonbuk National University, Iksan 54596, Korea.

出　　处：《The Journal of Biomedical Research》2017年第5期453-461,共9页生物医学研究杂志（英文版）

基　　金：supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A6A3A04063769)

摘　　要：Difructose dianhydride Ⅳ(DFA-Ⅳ) is produced from levan, which is a natural polysaccharide that belongs to the fructan family, through the activity of levan fructotransferase(LF) derived from microorganisms. Recently, DFA-Ⅳ has been expected to have diverse applications in the food and medical industry. Here, we examined the potential application of DFA-Ⅳ for in vitro fertilization(ⅣF) in pigs. In the assessment of acrosomal integrity during incubation, intact acrosomal or viable spermatozoa were highly sustained in 0.1% or 0.25% DFA-Ⅳ(69.8%-70.8%,P<0.05). Reactive oxygen species(ROS) levels during sperm incubation decreased following the addition of DFA-Ⅳ, and 0.1 %-0.5% DFA-Ⅳ in particular significantly decreased ROS production relative to that seen with no addition or 0.75% DFA-Ⅳ. Total fertilization(mono + polyspermic oocyte) rate was significantly higher in the addition of0.1% DFA-Ⅳ(94.2%) than with other concentrations(71.8%-86.7%, P<0.05). When using reduced ⅣF times and lower sperm numbers, we found that addition of 0.1%-0.5% DFA-Ⅳ significantly increased the fertilization rate(P<0.05). Fertilized oocytes treated with 0.1% DFA-Ⅳ exhibited higher embryonic development and blastocyst formation than those treated with other concentrations(P<0.05). Consequently, the addition of DFA-Ⅳ during ⅣF improved fertilization and embryonic development, suggesting the possible use of novel sugars for enhancement of assisted reproductive technology(ART) in mammals.Difructose dianhydride Ⅳ(DFA-Ⅳ) is produced from levan, which is a natural polysaccharide that belongs to the fructan family, through the activity of levan fructotransferase(LF) derived from microorganisms. Recently, DFA-Ⅳ has been expected to have diverse applications in the food and medical industry. Here, we examined the potential application of DFA-Ⅳ for in vitro fertilization(ⅣF) in pigs. In the assessment of acrosomal integrity during incubation, intact acrosomal or viable spermatozoa were highly sustained in 0.1% or 0.25% DFA-Ⅳ(69.8%-70.8%,P<0.05). Reactive oxygen species(ROS) levels during sperm incubation decreased following the addition of DFA-Ⅳ, and 0.1 %-0.5% DFA-Ⅳ in particular significantly decreased ROS production relative to that seen with no addition or 0.75% DFA-Ⅳ. Total fertilization(mono + polyspermic oocyte) rate was significantly higher in the addition of0.1% DFA-Ⅳ(94.2%) than with other concentrations(71.8%-86.7%, P<0.05). When using reduced ⅣF times and lower sperm numbers, we found that addition of 0.1%-0.5% DFA-Ⅳ significantly increased the fertilization rate(P<0.05). Fertilized oocytes treated with 0.1% DFA-Ⅳ exhibited higher embryonic development and blastocyst formation than those treated with other concentrations(P<0.05). Consequently, the addition of DFA-Ⅳ during ⅣF improved fertilization and embryonic development, suggesting the possible use of novel sugars for enhancement of assisted reproductive technology(ART) in mammals.

关 键 词：difructose dianhydride-Ⅳ SPERMATOZOA in vitro fertilization EMBRYO PIG 

分 类 号：R714.8[医药卫生—妇产科学]