清咽爽喉方的质量标准研究  

A study of quality standard for Qingyan Shuanghou prescription

作　　者：李永祝[1] 文远大[1] 余俊[1] 彭平健 杨贤军[1] 

机构地区：[1]湖南省怀化市第一人民医院,湖南怀化418000

出　　处：《湖南中医杂志》2017年第9期170-172,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药管理局资助项目(编号:201586)

摘　　要：目的:建立清咽爽喉方的质量标准。采用薄层色谱法(TLC)对清咽爽喉方中的麦冬、射干进行定性鉴别,以高效液相色谱法(HPLC)对菊花中的有效成分绿原酸、木犀草苷进行含量测定。方法:色谱柱:用十八烷基硅烷键合硅胶为填充剂C18(4.6mm×250mm,5μm);以乙腈为流动相A,以0.1%磷酸溶液为流动相B;检测波长为348nm。流速:1.0m L/min;柱温25℃。结果:清咽爽喉方的麦冬、射干TCL中,在与对照药材色谱相应的位置上,显相同颜色的斑点;绿原酸在7.5~750μg范围内线性关系良好,R=0.9998,木犀草苷在5.35~535μg范围内线性关系良好,R=0.9999,平均加样回收率分别为96.69%、98.47%,RSD分别为0.99%、1.62%。结论:本方法准确可行,可作为本品的质量控制。Objective：To investigate the quality standard for Qingyan Shuanghou prescription,to perform qualitative i- dentification of Radix Ophiopogonis and blackberry lily rhizonle in Qingyan Shuanghou prescription by thin layer chroma- tography（TLC） , and to determine the content of effective constituents chlorogenic acid and cynaroside in chrysanthemum by high - performance liquid chromatography （ HPLC ）. Methods ： HPLC was performed on a C18 column with octadecylsilane chemically bonded silica gel as the filler（4.6mm ×250mm,5μm）with a mobile phase of acetonitrile （A） -0. 1% phos- phoric acid solution （B）at a detection wavelength of 348 nm, a flow rate of 1.0mL/min, and a column temperature of 25℃. Results：TLC of Radix Ophiopogonis and blackben^y lily rhizome in Qingyan Shuanghou prescription obtained the spots with the same color at the same positions as TLC of the control medicinal material. Chlorogenic acid showed a good linear relationship within the range of 7.5 -750μg（ r = 0. 9998 ） ,with an average recovery rate of 96.69% （relative stand- ard deviation [ RSD~ = 0. 99% ）. Cynaroside showed a good linear relationship within the range of 5.35 -535μg（ r = 0. 9999 ） , with an average recovery rate of 98.47% （RSD = 1.62% ）. Conclusion：This method is accurate and feasible and can be used for quality control of this product.

关 键 词：清咽爽喉方 TLC HPLC 质量标准 

分 类 号：R285.5[医药卫生—中药学]