广东省中山地区蜚蠊中共生菌沃尔巴克氏体的监测与分析  

Monitoring and analysis of symbiotic Wolbachia in cockroaches at Zhongshan area

作　　者：陈佩 陈健[1] 邱德义 何秋星 

机构地区：[1]中山出入境检验检疫局检验检疫技术中心,广东中山528403 [2]广东药科大学,广州510006

出　　处：《中国媒介生物学及控制杂志》2017年第5期422-427,共6页Chinese Journal of Vector Biology and Control

基　　金：国家质量基础项目(NQI;2016YFF0203205);国家质量监督检验检疫总局科技计划项目(2015IK069);广东省国际合作项目(2015A050502009)~~

摘　　要：目的了解中山地区蜚蠊中沃尔巴克氏体的感染状况及其基因型别。方法于2015年6月至2016年7月,采集广东省中山市的常见蜚蠊种类,通过扩增沃尔巴克氏体的wsp基因并测序判断其是否感染,同时进行系统进化分析;用配对设计t检验分析不同种类雌雄个体感染率差异是否有统计学意义。结果共检测12种154只蜚蠊样本,其中5种42只蜚蠊检出感染沃尔巴克氏体,个体感染率为27.27%,且雌雄感染率差异无统计学意义(|t|=0.542,|t|<t_(0.01(4))=2.776,P>0.01)。共获得8个wsp基因单倍型序列,其分别属于wAlbB、wAlbD和wAlbF型沃尔巴克氏体。结论首次发现维丽斯璐蠊、中华玛啦蠊、姬蠊科sp.、玛蠊属sp.1和玛蠊属sp.2携带沃尔巴克氏体,其基因型属于wAlbB、wAlbD和wAlbF型,且可能在维丽斯璐蠊体内检测到1种新的wAlbF基因型的沃尔巴克氏体。Objective To understand Wolbachia infection and its genotype in the cockroaches at Zhongshan area.Methods Cockroaches were collected in Zhongshan of Guangdong province between June 2015 to July 2016.wsp genes of Wolbachia were amplified and sequenced to identify if the cockroaches were infected or not,phylogenetic analysis of the wsp in these cockroaches was conducted as well.Paired t test was applied to the analysis of infection difference between the females and males.Results A total of 42 cockroaches belonging to 5 species were infected with Wolbachia out of all the studied 154 cockroach individuals of 12 species,the infection rate of Wolbachia was 27.27%at individuals level,and the difference of Wolbachia infection rate between males and females wasn＆#39;t significant.There were 8 wsp haplotype sequences were classified as wAlbB,wAlbD,and wAlbF based on the phylogenetic analysis.Conclusion Wolbachia infection in Lupparia vilis,Malaccina sinica,Margatta sp.1,Margatta sp.2,and Ectobiidae sp.were detected for the first time in the world,those genotypes belong to wAlbB,wAlbD,and wAlbF.Among those wsp haplotype sequences,there might be a new wAlbF Wolbachia type in L.vilis.

关 键 词：蜚蠊 沃尔巴克氏体 WSP基因 

分 类 号：R384.9[医药卫生—医学寄生虫学] R376[医药卫生—基础医学]