Ang-1基因转染的内皮祖细胞移植对大鼠肾脏缺血再灌注后的保护作用  

Ang-1 promotes homing of endothelial progenitor cells on renal ischemia and reperfusion injury in male rats

作　　者：薛建新[1,2] 李潇 贾瑞鹏[4] 秦志强[2] 

机构地区：[1]东南大学附属第二医院泌尿外科,江苏南京210003 [2]南京医科大学第一附属医院,江苏南京210029 [3]南京医科大学附属肿瘤医院,江苏南京210009 [4]南京医科大学附属南京医院,江苏南京210006

出　　处：《国际泌尿系统杂志》2017年第5期755-760,共6页International Journal of Urology and Nephrology

摘　　要：目的 评估Ang-1基因转染的内源性内皮祖细胞（endothelial progenitor cells,EPCs）移植治疗肾脏缺血再灌注损伤的作用,进一步完善EPCs对缺血肾脏产生保护作用的旁分泌机制.方法利用重组腺病毒载体Ad-Ang-1感染EPCs,并进行转染效率鉴定,进一步移植治疗大鼠缺血肾脏,术后72 h给予肾功能评估,并检测大鼠缺血肾脏中VEGF、Ang-1以及Ang-2表达情况.结果EPCs移植治疗后,大鼠肾脏功能明显改善,术后72 h检测大鼠患肾,其内VEGF、Ang-1以及Ang-2表达均明显提升.结论 Ang-1基因转染的EPCs移植治疗可以有效地治疗大鼠肾脏IRI,其作用机制可能与EPC归巢后旁分泌过量Ang-1相关,并且促使肾脏血管新生.Objective To assess whether angiopoietin-1 （Ang-1） promoted the homing of endothelial progenitor cells （EPCs） to protect kidneys from ischemia/reperfusion injury （IRI） in male rats.Methods Recombinant adenovirus encoding Ang-1 gene （Ad-Ang-1） was used to infect EPCs,and the transfection efficiency was identified.Male Sprague-Dawley rats were injected EPCs suspension liquid which was transfected by Ad-Ang-1 from the femoral vein of rats after the operation method of IRI-operated group.At 24 h and 72 h after reperfusion,serum samples were respectively collected for renal function.Besides,kidney tissues were harvested to observe renal morphology changes.Subsequently,vascular endothelial growth factor （VEGF）,Ang-1 and angiopoietin-2（Ang-2） expression levels in the kidneys were measured with quantitative real-time PCR and Western Blot at the indicated time points after reperfusion.Results The renal function was significantly improved,after EPCs transplantation with Ad-Ang-1.At 72 hours after reperfusion,expression levels of VEGF,Ang-1 and Ang-2 in the kidneys of EPCs-treated rats were significantly increased.Conclusions EPCs transplantation with Ad-Ang-1 in the treatment of kidney IRI in rats is effective.In addition,Ang-1 would play important roles in the protective effect of homing of EPCs on renal acute IRI,and further promote renal angiogenesis.

关 键 词：再灌注损伤 肾 转染 干细胞 

分 类 号：R692[医药卫生—泌尿科学]