异常黑胆质成熟剂对异常黑胆质型肝癌病证大鼠模型候选基因表达的影响  

Identification of differentially expressed genes in an abnormal savda rat model of hepatocarcinoma treated with abnormal Savda munziq

作　　者：娜孜拉木.玉苏甫江 王延蛟[1] 祖力皮喀尔.阿卜杜热合曼 古丽尼格尔.雪合拉提 斯坎德尔.白克力 Nazilamu·Yusufujiang Yan-Jiao Wang Zulipikaer·Abudureheman Gulinigeer·Xuehelati Sikandeer·Baikeli(Department of Biochemistry, Basic Medical College, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China)

机构地区：[1]新疆医科大学基础医学院生物化学教研室,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《世界华人消化杂志》2017年第27期2449-2458,共10页World Chinese Journal of Digestology

基　　金：国家自然科学基金;Nos.81560805;81160545~~

摘　　要：目的探讨异常黑胆质成熟剂(abnormal savda munziq,ASM)对异常黑胆质型肝癌病证大鼠模型候选基因表达水平的影响.方法选取♂清洁Wistar大鼠随机分为6个组.联合维吾尔医学理论及二乙基亚硝胺(diethylnitrosamine,DEN)诱导建立异常黑胆质型肝癌病证大鼠模型,并用ASM高(6.0 g/kg),中(3.0 g/kg),低(1.5 g/kg)不同剂量对模型组全程干预20 wk,观察肝脏组织形态学变化,并通过RT-qPCR验证表达谱芯片筛选出的部分差异表达候选基因.结果观察肝脏组织形态学变化发现,异常黑胆质型肝癌病证模型组成癌率明显高于对照肝癌组,ASM干预组成癌率明显低于异常黑胆质型肝癌病证模型组;芯片结果显示,与对照肝癌组比较,异常黑胆质型肝癌病证模型组上调表达基因438种、表达下调基因451种,对从异常黑胆质型肝癌病证模型组与ASM干预组表达变化相反的11种基因中选出6种进行RT-qPCR验证,结果发现,在大部分组间mRNA表达水平有差异并具有统计学意义(P<0.01,P<0.05).结论STAT3、CyclinD1、EGLN3、EMP1、NEFL、IGFALS基因可能是ASM抗癌的作用靶点.AIM To identify the differentially expressed genes in an abnormal savda rat model of hepatocarcinoma treated with abnormal savda munziq（ASM）.METHODS Male Wistar rats were randomly divided into six groups. An abnormal savda rat model of hepatocarcinoma was established with diethylnitrosamine（DEN） according to Uyghur medicine theory. Different doses of ASM（6.0, 3.0, and 1.5 g/kg） were used to intervene the model rats for 20 wk. The histological changes in hepatocarcinoma tissue were observed. The genes with differential expression were screened, several of which were investigated by RT-qPCR.RESULTS The histology of liver tissue showed that the cancerogenic rate was significantly higher in rats with abnormal savda than in those without. Cancerogenic rates in the ASM intervention groups were significantly lower than those in rats with abnormal savda. Compared with the control group, 438 genes were up-regulated and 451 down-regulated in abnormal savda rats with hepatocarcinoma. Among these differentially expressed genes, 11 showed converse expression patterns between the hepatocarcinoma group and ASM intervention groups. RT-qPCR verified that STAT3, CyclinD1, EGLN3, EMP1, NEFL, and IGFALS were differentially expressed in different groups（P 0.01 or P 0.05）.CONCLUSION STAT3, CyclinD1, EGLN3, EMP1, NEFL, and IGFALS may be involved in the anti-cancer effects of ASM.

关 键 词：异常黑胆质成熟剂 肝癌 候选基因 基因表达 

分 类 号：R29[医药卫生—民族医学]