Immune fluorescence test strips based on quantum dots for rapid and quantitative detection of carcino-embryonic antigen  

作　　者：Yudong Wu Weipan Peng Qian Zhao Jiafang Piao Bo Zhang Xiaoli Wu Hanjie Wang Zhihong Shi Xiaoqun Gong Jin Chang 

机构地区：[1]School of Life Sciences,School of Materials Science and Engineering,Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology(Tianjin) [2]Department of Neurology,and Tianjin Neurosurgery Institute,Tianjin Huanhu Hospital

出　　处：《Chinese Chemical Letters》2017年第9期1881-1884,共4页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51373117,51303126 and 31600800);Tianjin Natural Science and Technology Foundation(No.16ZXMJSY00010)

摘　　要：At present,many researchers focused on the point-of-care testing（POCT）,a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection.Herein,we developed a newly QD-immune fluorescence test strips（QD-IFTS） based on quantum dots（QDs） as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen（CEA）,a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis.And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS.Under the optimized reaction conditions,chromatographic time of the newly QD-IFTS was only25 min,sample volume of the newly QD-IFTS was only 40 m L and the LOD of the newly QD-IFTS was 0.72 ng/m L.In addition,the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples,and the results revealed great potential in clinical POCT of other biomarkers.At present,many researchers focused on the point-of-care testing（POCT）,a method of disease markers detection without large-scale instruments and specialized persons.However,most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection.Herein,we developed a newly QD-immune fluorescence test strips（QD-IFTS） based on quantum dots（QDs） as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen（CEA）,a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis.And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS.Under the optimized reaction conditions,chromatographic time of the newly QD-IFTS was only25 min,sample volume of the newly QD-IFTS was only 40 m L and the LOD of the newly QD-IFTS was 0.72 ng/m L.In addition,the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples,and the results revealed great potential in clinical POCT of other biomarkers.

关 键 词：Point-of-care testing Immunochromatography assay Quantum dots Carcinoembryonic antigen Quantitative detection 

分 类 号：O657.3[理学—分析化学] R730.43[理学—化学]