青风藤水煎液对蟾蜍坐骨神经干电生理的影响  

Effects of Caulis Sinomenii decoction on electrophysiological character of sciatic nerve trunk of toad

作　　者：王波[1] 

机构地区：[1]西安文理学院生物与环境工程学院基因工程实验室,陕西西安710065

出　　处：《中国应用生理学杂志》2017年第4期377-379,共3页Chinese Journal of Applied Physiology

基　　金：西安市科技计划项目-西安文理学院科研创新基金专项项目(CXY1531WL17)

摘　　要：目的:探索青风藤对蟾蜍离体坐骨神经干电生理特性的影响。方法:40只蟾蜍随机分成4组:任氏液对照组和青风藤低、中、高剂量组(0.2、0.10、0.05 g/ml)(n=10),通过RM6240C多道生理信号采集处理系统记录其在不同浓度青风藤水煎分别浸泡15 min和30 min时,动作电位的传导速度、幅度和动作电位阈强度。结果:与对照组相比,高剂量组的神经干动作电位的传导速度显著减慢(P<0.01),中、高剂量组的幅度显著降低(P<0.01),高剂量组的动作电位阈强度显著增大(P<0.01)。结论:动作电位传导速度、幅度与青风藤剂量呈负相关,动作电位阈强度与青风藤剂量呈正相关。青风藤水煎液降低坐骨神经的兴奋性,阻滞动作电位的传导,可能发挥抑制坐骨神经痛的作用。Objective： To investigate the effects of Caulis Sinomenii（ traditional Chinese medicine） on electrophysiological character of sciatic nerve trunk of toad. Methods： Forty toads were randomly divided into four groups： Ringer＇s solution（ as a control group） and three experimental groups were treated with 0.20,0.10,0.05 g/ml Caulis Sinomenii decoction,each group was soaked for15 and 30 min respectively. The conduction velocity,amplitude and threshold intensity of the sciatic nerves from each group were measured by RM6240 C Physiological signals recording system. Results： Compared with the control group,the conduction velocity in high dose group was significantly decreased（ P0.01）,the amplitude of the action potential in medium and high dose groups were lower（ P 0.01）; but the threshold intensity of the nerve trunk action potential in high dose group was increased significantly compared with that in control group（ P0.01）. Conclusion： The Caulis Sinomenii decoction can reduce the excitability sciatic nerve stem of toad and block the conduction of the action potential,which may play important roles in inhibiting of sciatica.

关 键 词：青风藤 坐骨神经 动作电位 蟾蜍 

分 类 号：Q422[生物学—神经生物学]