失重下骨碎补总黄酮经ERK通路促成骨细胞增殖研究  

Proliferation of OB Intervened by TFDF through ERK pathway under Weightless Condition

作　　者：尹文哲[1] 邢成亮 王建业[2] 孙奇峰[1] 吕成倩[1] 褚艳杰[1] YIN Wen - zhe XING Cheng - liang WANG Jian - ye SUN Qi - feng LV Cheng - qian CHU Yan - jie(The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China)

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086 [2]佳木斯大学附属第一医院,黑龙江佳木斯154002

出　　处：《中医药学报》2017年第5期45-48,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金面上项目(81374070);全国临床医药研究专项基金重点项目研究(L2012051)

摘　　要：目的:观察失重下骨碎补总黄酮对成骨细胞增殖作用及ERK通路变化,探讨骨碎补总黄酮促成骨细胞增殖的可能机制。方法:培养SD仔鼠成骨细胞,建立微重力模型,加高、中、低剂量骨碎补血清培,观察细胞形态学变化,PNPP检测成骨细胞ALP变化,MTT检测成骨细胞增殖。PCR测定ERK通路。结果:与空白对照组和低剂量组相比,中、高剂量组细胞增殖能力及ALP活性值均升高,ERK通路表达升高具有统计学意义(P<0.05),与阳性对照组接近。结论:骨碎补在失重下可促成骨细胞增殖,其机制与ERK通路激活相关。Objective ： To tion, and to explore its observe the effect of TFDF on possible mechanism. Methods ： osteoblasts （OB） through ERK pathway under weightless condi- OB from immature rats were incubated, and different concentra- tion of TFDF was given under micro - gravity condition. Changes of cell morphology were observed, ALP change was de- tected by PNPP, and proliferation of OB was observed by MTT. Expression of ERK pathway was tested with PCR. Re- suits： Proliferation ability, ALP value, and ERK expression were all significantly increased in the high - dose TFDF group and the medium - dose TFDF group, compared to those in the low - dose group and the blank control group, which were statistically significant（P 〈0.05 ） ; and the elevation was close to that of the positive control group. Conclu- sion： TFDF can promote the OB proliferation, which may be related to the activation of ERK pathway expression.

关 键 词：失重 骨碎补 胞外信号调节激酶 成骨细胞 

分 类 号：R285.5[医药卫生—中药学]