基于谱效关系的钩吻炮制减毒存效的实验研究  

Experimental Study on the Toxicity Attenuation and Efficacy Reservation of Pueraria Iobata Based on Spectrum-effect Relation

作　　者：吴水生[1] 李德森[1] 许豪然 廖华军[1] 陈亮[3] 李丹[3] WU Shui- sheng LI De- sen XU Hao- ran LIAO Hua- jun CHEN Liang LI Dan(Fujian University of TCM, Fuzhou 350122, China North Malaysia TCM College, Penang 13200, Malaysia Fuzhou TCM Hospital Affiliated to Fujian University of TCM, Fuzhou 350001, China)

机构地区：[1]福建中医药大学,福建福州350122 [2]马来西亚北马中医药学院,马来西亚槟城13200 [3]福建中医药大学附属福州市中医院,福建福州350001

出　　处：《中医药学报》2017年第5期80-84,共5页Acta Chinese Medicine and Pharmacology

基　　金：福州市科技局资助项目(2014-S-140-3)

摘　　要：目的:研究砂炒钩吻的减毒存效的工艺及镇痛药效。方法:采用烘烤箱高温加热法,以镇痛药效、急性毒性为评价指标,在单因素试验基础上,选择炮制时间、炮制温度为自变量,采用星点设计-响应面法优选钩吻炮制工艺的研究。结果:钩吻在190~230℃温度下,经过10~60 min的炮制后,毒性明显降低,且保留一定的镇痛效应。对炮制品的外观、毒性和镇痛药效综合考虑,在炮制温度200~220℃,炮制时间在10~20 min内进行最佳工艺优选,得到钩吻的最佳炮制工艺:炮制温度210℃,炮制时间15 min。在此条件下进行验证性实验,结果与实验预测值较为接近,说明采用响应面法得到的工艺参数可靠,具有一定的实用价值。结论:砂炒钩吻炮制后毒性明显降低,同时又保留良好的镇痛药效,取得了良好的预期结果。Objective： To study the toxicity attenuation and efficacy reservation of Pueraria Iobata processed by hot sand frying. Methods： Analgesic effect and acute toxicity were taken as evaluation index to select the preferable process by u- sing oven heating method, with processing time and processing temperature as the independent variable in central com- posite design - response surface test. Results ： The toxicity was significantly reduced, and certain analgesic effect was re- tained in Pueraria lobata under 190 - 230 ℃ after 10 -60 min of preparation. After comprehensive consideration, the optimized process was narrowed down to temperature of 200 - 220 ℃ and time 10 -20 min after. The best process was finally confirmed at temperature of 210℃, processing time was 15min. Under this condition, the experimental results were in good agreement with the experimental predictions, which showed that the process parameters obtained by re- sponse surface method were reliable and had practical value. Conclusion： Hot sand frying can attenuate the toxicity and preserve analgesic effect of Pueraria lobata.

关 键 词：钩吻 炮制 减毒存效 星点设计-响应面法 

分 类 号：R283.1[医药卫生—中药学]