巴西橡胶树WRKY转录因子基因HbWRKY41的克隆与表达分析  

Cloning and Expression Analysis of a WRKY Transcription Factor Gene HbWRKY41 in Hevea brasiliensis

作　　者：刘辉[1] 韦永选 邓治[1] 代龙军[1] 杨洪[1] 李德军[1] LIU Hui WEI Yongxuan DENG Zhi DAI Longjun YANG Hong LI Dejun(Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Biology and Genetic Resources of Rubber Tree ,Ministry of Agriculture,Danzhou 571737 ,China)

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737

出　　处：《华北农学报》2017年第5期91-96,共6页Acta Agriculturae Boreali-Sinica

基　　金：中国热带农业科学院基本科研业务费专项(1630022017028;1630022014006)

摘　　要：为阐明巴西橡胶树WRKY转录因子基因Hb WRKY41的分子特征及表达特性,利用RT-PCR方法从巴西橡胶树中克隆了该基因。Hb WRKY41开放阅读框1 020 bp,编码339个氨基酸,预测蛋白分子量为38.48 k Da,理论等电点为5.52。Hb WRKY41含有1个WRKY保守结构域和1个C_2HC型锌指结构,与蓖麻、棉花、大豆及拟南芥WRKY41蛋白聚为一类,属于Ⅲ类WRKY转录因子家族成员。实时荧光定量PCR检测结果表明,Hb WRKY41在巴西橡胶树根、树皮、胶乳、叶和花等组织中均有表达,以在衰老叶片中的表达最高,而在胶乳和树皮中的表达相对较低。干旱、低温及高盐胁迫均可上调Hb WRKY41的表达,其中以低温或盐胁迫下Hb WRKY41的表达持续升高。Hb WRKY41可能在巴西橡胶树叶片衰老和非生物胁迫应答中起着重要调控作用。To reveal the molecular characterization and expression patterns of the WRKY transcription factor gene HbWRKY41 in Hevea brasiliensis（ para rubber tree）,this gene was cloned from para rubber tree by RT-PCR.HbWRKY41 contained an open reading frame of 1 020 bp encoding a protein of 339 amino acids. The molecular weight and isoelectric point of Hb WRKY41 were 38. 48 k Da and 5. 52,respectively. Hb WRKY41 possessed a conserved WRKY domain and a C_2HC-type zinc-finger motif,and it was clustered into the group Ⅲ of the WRKY family with WRKY41 proteins from castor,cotton,soybean and Arabidopsis thaliana. Quantitative Real-time PCR analysis showed that HbWRKY41 was expressed in root,bark,latex,leaf and flower of para rubber tree,with the highest expression in senescent leaf and relatively low expression in latex and bark. HbWRKY41 expression was up-regulated by cold,drought and salt stresses,its expression was continuously increased during cold or salt stress. HbWRKY41 may play important roles in regulating leaf senescence and abiotic stress responses in para rubber tree.

关 键 词：巴西橡胶树 WRKY转录因子 基因克隆 表达分析 非生物胁迫 

分 类 号：Q78[生物学—分子生物学] S794.03[农业科学—林木遗传育种]