丹顶鹤体内曲领棘缘吸虫的鉴定  

Identification of Echinoparyphium recurvatum in Grus japonensis

作　　者：王丽坤[1] 张莹 崔宇超[1] 高俊峰[1,2] 侯美如[1] 杨淑萍[1] Wang Likun Zhang Ying Cui Yuchao Gao Junfeng Hou Meiru Yang Shuping(Dapartment of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar 161000 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University)

机构地区：[1]黑龙江省兽医科学研究所,齐齐哈尔161000 [2]黑龙江八一农垦大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2017年第5期37-39,105,共4页journal of heilongjiang bayi agricultural university

摘　　要：为了鉴定经吡喹酮治疗有拉稀症状的丹顶鹤排出的吸虫种类,应用形态学结合分子生物学方法对该吸虫进行虫种鉴定。结果显示:该虫体形态与文献中报道的曲领棘缘吸虫的形态一致;所分离的吸虫ITS r DNA序列全长为1 083 bp,通过与NCBI上其他棘口科吸虫的ITS序列进行比对,表明该虫体与Echinostoma trivolvis的同源性可高达93%,属于棘口属吸虫,得出虫体鉴定结果为曲领棘缘吸虫,为鱼媒介寄生虫的防治提供参考依据。In order to identification the trematode species,which were collected from Grus japoner-sis with diarrhea,morphology and molecular hiology method were carried out. Morphological identification results showed that the morphological characteristscs were consistent with Echinoparyphium recurvatum. Molecular identification showed,the length of ITS rDNA sequence was 1 083 bp long, Comparison ITS sequence of NCBI morphological,it displayed that the highest similarity rate was 93% with Echinopar）phium recurvatum,which behmgs to Echinostoma members. Therefore,the trematode isolated from Grus jat-oner-sis was identified as Echinoparyphium recurvatum. The results provided a reference for the prevention of fish-born trematode.

关 键 词：丹顶鹤 形态学 ITS序列 曲领棘缘吸虫 

分 类 号：S852.7[农业科学—基础兽医学]