TGF-β参与细胞内钙池操纵性Ca^(2+)内流调节FGF23的释放  

TGF-β-sensitive Store-operated Ca^(2+) Entry in the Regulation of FGF23 Release

作　　者：张冰冰[1] 杨威[2] 徐闯[2] 夏成[2] 张洪友[2] 王春仁[1,2] 余丽芸[1] Zhang Bingbing Yang Wei Xu Chuang Xia Cheng Zhang Hongyou Wang Chunren Yu Liyun(College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319 College of Animal Science and Technology, Heilongjiang Bayi Agricultural University)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]黑龙江八一农垦大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2017年第5期66-68,73,共4页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金青年项目(31502133);黑龙江省科学基金面上项目(C2015043)

摘　　要：为了研究TGF-β是否通过细胞内钙池操纵性Ca^(2+)内流来调节FGF23的释放,通过TGF-β处理大鼠骨肉瘤细胞利用荧光定量PCR技术检测细胞内FGF23和Orai1基因的表达。结果表明,与正常对照组细胞比较,TGF-β处理组FGF23与Orai1的基因表达水平显著升高,并且Orai1和NF-κB的抑制剂能够拮抗TGF-β的作用。表明TGF-β通过NF-κB/Orai1调节FGF23的释放,为进一步治疗慢性肾病等疾病提供依据。To study whether the effect of TGF-β on FGF23 mRNA expression was involved and required Ca^2＋ entry, FGF23 and Orail mRNA were measured by real-time RT-PCR in UMR106 cells. The results showed that FGF23 mRNA expression was significantly enhanced by TGF-β treat in UMR106;conversely,an effect was abolished by Orail inhibitor YM58483 and NF-κB inhibitor Wogonin. Moreover, Orail transcript levels were significantly up-regulated by TGF-β,an effect attenuated by wogonin. It indicated TGF-β up-regulates FGF23 was released by NF-κB dependent up-regulation of Orail transcription,which may give strategies for new therapeutic options.

关 键 词：TGF-Β 成纤维生长因子23 细胞内钙池操纵性Ca^2+内流 

分 类 号：Q25[生物学—细胞生物学]