miR-218-1-3p对非小细胞肺癌细胞增殖、周期及凋亡的影响  

Effects of miR-218-1-3p on Cell Proliferation,Cycle,and Apoptosis of Non-small Cell Lung Cancer

作　　者：栾岚[1] 张天戈[2] 韩斌[2] 罗文婷[2] 吴非[1] 廖鑫[1] 张君杰 白阳[1] 程新宇[1] 滕猛[1] 王翠芳[1] 

机构地区：[1]沈阳医学院附属中心医院病理科,沈阳110024 [2]中国医科大学附属盛京医院泌尿外科,沈阳110004

出　　处：《中国医科大学学报》2017年第11期980-983,994,共5页Journal of China Medical University

基　　金：国家自然科学基金(81401907);沈阳医学院青年基金(20102029)

摘　　要：目的探究miR-218-1-3p对非小细胞肺癌A549细胞周期和凋亡的影响。方法使用LipofectamineTM 2000 Reagent将miR-218-1-3p模拟物转染入非小细胞肺癌A549细胞中,实时PCR检测细胞中miR-218-1-3p的表达,MTS法检测miR-218-1-3p对A549细胞增殖的影响,流式细胞仪检测转染miR-218-1-3p的A549细胞周期及凋亡的改变,荧光定量PCR检测细胞增殖、周期和凋亡相关指标变化。结果同对照组相比,miR-218-1-3p模拟物组细胞生长受到明显抑制(P<0.05),S期和G2-M期细胞比例明显降低(P<0.05),此外miR-218-1-3p模拟物组早期凋亡细胞比例较对照组明显增加(P<0.05)。继续检测了与细胞增殖、周期和凋亡相关的指标,其中CYCLIN-D1和BCL-2的表达显著下调。结论 miR-218-1-3p可能通过调控CYCLIN-D1和BCL-2,从而抑制非小细胞肺癌A549细胞的增殖,对其产生细胞周期阻滞并促进其凋亡。Objective To investigate the effect of miR-218-1-3p on the proliferation, cycle, and apoptosis of A549 cells in non-small-cell lung cancer. Methods miR-218-1-3p was transfected into non-small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent,and the expression of miR-218-3p was detected by real-time PCR. Invasion and migration were assayed using the Transwell method. The effect of miR-218-1-3p on the proliferation of A549 cells was assayed by the MTS method. Changes in the cell cycle and apoptosis of A549 cells transfeeted with miR-218-1-3p was detected by flow eytometry. Changes in indicators related to cell proliferation, cycle, and apoptosis were detected by fluorescence quantitative PCR. Results Compared to the control group,the cell proliferation of A549 cells was significantly inhibited （P 〈 0.05） and the proportion of cells in the S and G2-M phases was significantly decreased when miR-218-1-3p was up-regulated. In addition, compared with the control group, the early apoptotic rate was significantly increased by up-regulating miR-218-1-3p. We further detected indicators related to cell proliferation,cycle,and apoptosis and found that CYCLIN-D1 and BCL-2 were significantly down-regulated. Conclusion miR-218-1-3p may inhibit proliferation, induce cell cycle arrest, and promote cell apoptosis of non-small cell lung cancer A549 cells by regulating CYCLIN-D 1 and BCL-2.

关 键 词：MI R-218-1-3p 非小细胞肺癌 增殖 周期 凋亡 

分 类 号：R34[医药卫生—基础医学]