实时荧光核酸恒温扩增方法检测男性生殖道感染的临床观察  

Clinical Study of Male Urogenital Infection by Simultaneous Isothermal Amplification Assay

作　　者：肖秀美[1] 李昂[1] 马啸龙 刘宠 李晶[1] 郑佳佳[1] 姚贝[1] 

机构地区：[1]北京大学第三医院检验科,北京100191 [2]北京大学第三医院输血科,北京100191

出　　处：《中国皮肤性病学杂志》2017年第11期1217-1219,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的分析男性泌尿生殖道感染患者解脲脲原体(Ureaplasma urealyticum,UU)、淋病奈瑟菌(Neisseria gonorrhoeae,NG)、沙眼衣原体(Chlamydia trachomatis,CT)的检出特征。方法采用实时荧光核酸恒温扩增技术检测2015年5月-2016年4月本院门诊泌尿生殖道感染男性患者尿液中的UU,CT和NG,按年龄和诊断进行细化分层研究。结果 2172例患者中,UU,CT和NG阳性率分别为27.12%、9.67%和3.96%,20岁以下人群NG阳性率最高(8.33%),尿道炎患者的CT,NG阳性率最高(14.77%和7.03%),不育症患者UU阳性率最高(26.89%)。结论 CT,NG和UU是男性生殖道感染的重要病原体,NG和UU检出率有升高趋势,宜加强监测。Objective To characterize the prevalence ofUreaplasma urealyticum（UU）, Neisseria gonorrhoeae（NG）and Chlamydia trachomatis（CT）in male outpatients with suspected urogenital infections.Methods The simultaneous isothermal amplification assay was applied to detect the three pathogens in first catch urine collected from May 2015 to Apr 2016, the prevalence were stratified by age and diagnosis.Results A total of 2172 samples were collected in a year,the positive rates of UU, CT and NG were 27.12%, 9.67% and 3.96%.The positive rate of NG among patients less than 20 years old was the highest（8.33%）.The positive rates of CT and NG in patients with urethritis were the highest（14.77% and 7.03%）; the prevalence of UU in patients complaining infertility was the highest（26.89%）.Conclusion CT, NG and UU are the important pathogens for male urogenital infection, the prevalence of NG and UU increased significantly, the detection should be placed great emphasis for the higher prevalence.

关 键 词：沙眼衣原体 淋病奈瑟菌 解脲脲原体 实时荧光核酸恒温扩增技术 生殖道感染 

分 类 号：R691.3[医药卫生—泌尿科学]